HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Dissociation of seizure traits in inbred strains of mice using the flurothyl kindling model of epileptogenesis.

Abstract
Previous seizure models have demonstrated genetic differences in generalized seizure threshold (GST) in inbred mice, but the genetic control of epileptogenesis is relatively unexplored. The present study examined, through analysis of inbred strains of mice, whether the seizure characteristics observed in the flurothyl kindling model are under genetic control. Eight consecutive, daily generalized seizures were induced by flurothyl in mice from five inbred strains. Following a 28-day rest period, mice were retested with flurothyl. The five strains of mice demonstrated inter-strain differences in GST, decreases in GST across seizure trials, and differences in the behavioral seizure phenotypes expressed. Since many of the seizure characteristics that we examined in the flurothyl kindling model were dissociable between C57BL/6J and DBA/2J mice, we analyzed these strains in detail. Unlike C57BL/6J mice, DBA/2J mice had a lower GST on trial 1, did not demonstrate a decrease in GST across trials, nor did they show an alteration in seizure phenotype upon flurothyl retest. Surprisingly, [C57BL/6JxDBA/2J] F1-hybrids had initial GST on trial 1 and GST decreases across trials similar to what was found for C57BL/6J, but they did not undergo the alteration in behavioral seizure phenotype that had been observed for C57BL/6J mice. Our data establish the significance of the genetic background in flurothyl-induced epileptogenesis. The [C57BL/6JxDBA/2J] F1-hybrid data demonstrate that initial GST, the decrease in GST across trials, and the change in seizure phenotype differ from the characteristics of the parental strains, suggesting that these phenotypes are controlled by independent genetic loci.
AuthorsDominick Papandrea, Tara M Anderson, Bruce J Herron, Russell J Ferland
JournalExperimental neurology (Exp Neurol) Vol. 215 Issue 1 Pg. 60-8 (Jan 2009) ISSN: 1090-2430 [Electronic] United States
PMID18950623 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • Flurothyl
Topics
  • Age Factors
  • Analysis of Variance
  • Animals
  • Behavior, Animal (drug effects)
  • Disease Models, Animal
  • Epilepsy (chemically induced, pathology)
  • Flurothyl
  • Kindling, Neurologic (physiology)
  • Male
  • Mice
  • Mice, Inbred Strains
  • Phenotype
  • Reaction Time (drug effects)
  • Species Specificity

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: