Ganoderic acid Me inhibits tumor invasion through down-regulating matrix metalloproteinases 2/9 gene expression.

Abstract

The effect of ganoderic acid Me (GA-Me), which was purified from the fermentation mycelia of the traditional Chinese medicinal mushroom Ganoderma lucidum as reported (Tang W, Gu TY, Zhong JJ. Biochem Eng J. 2006;32:205-210), on anti-invasion was investigated. Wound healing assay indicated that GA-Me inhibited cell migration of 95-D, a human highly metastatic lung tumor cell line, in dose- and time-dependent manners. Results of cell aggregation and adhesion assays showed that GA-Me promoted cell homotypic aggregation and inhibited cell adherence to extracellular matrix (ECM). In addition, GA-Me suppressed matrix metalloproteinases 2/9 (MMP2/9) gene expressions at both mRNA and protein levels in 95-D cells according to qRT-PCR and Western blotting, respectively. The results demonstrated that GA-Me effectively inhibited tumor invasion, and it might act as a new MMP2/9 inhibitor for anti-metastasis treatment of carcinoma cells.

• Authors: Nian-Hong Chen, Jian-Wen Liu, Jian-Jiang Zhong
• Journal: Journal of pharmacological sciences (J Pharmacol Sci) Vol. 108 Issue 2 Pg. 212-6 (Oct 2008) ISSN: 1347-8613 [Print] Japan
• PMID: 18946196 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Matrix Metalloproteinase Inhibitors
• Protease Inhibitors
• RNA, Messenger
• Triterpenes
• ganoderic acid Me
• MMP2 protein, human
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Antineoplastic Agents, Phytogenic (pharmacology)
• Cell Adhesion (drug effects)
• Cell Aggregation (drug effects)
• Cell Line, Tumor
• Cell Movement (drug effects)
• Dose-Response Relationship, Drug
• Down-Regulation
• Gene Expression Regulation, Enzymologic (drug effects)
• Gene Expression Regulation, Neoplastic (drug effects)
• Humans
• Lung Neoplasms (enzymology, genetics, pathology)
• Matrix Metalloproteinase 2 (genetics, metabolism)
• Matrix Metalloproteinase 9 (genetics, metabolism)
• Matrix Metalloproteinase Inhibitors
• Neoplasm Invasiveness
• Protease Inhibitors (pharmacology)
• RNA, Messenger (metabolism)
• Time Factors
• Triterpenes (pharmacology)