Determination of metaldehyde in human serum by headspace solid-phase microextraction and gas chromatography-mass spectrometry.

Abstract

A rapid headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) method has been developed for the determination of metaldehyde in human serum samples. Metaldehyde is extensively used as a molluscicide for the control of slugs and snails, and cases of metaldehyde poisoning have been reported. Metaldehyde was headspace-extracted on a polydimethylsiloxane (PDMS) fiber at 70 degrees C for 25 min, desorbed, and analyzed rapidly by GC-MS. The method was validated for limit of detection (LOD), linearity, precision, and recovery. Although the recovery of the sample was very low, the method itself was rapid with a low detection limit of 0.25 microg/ml, R.S.D. value 12.6%, and linearity range 0.5-25.0 microg/ml (r(2)=0.999). The results demonstrated that the SPME-GC-MS method for the analysis of metaldehyde is simple, rapid, solvent-free, and does not require any pre-analysis conversions.

• Authors: Takeshi Saito, Seiji Morita, Megumi Motojyuku, Kazuki Akieda, Hiroyuki Otsuka, Isotoshi Yamamoto, Sadaki Inokuchi
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 875 Issue 2 Pg. 573-6 (Nov 15 2008) ISSN: 1570-0232 [Print] Netherlands
• PMID: 18945651 (Publication Type: Case Reports, Journal Article)

Chemical References

• Dimethylpolysiloxanes
• Molluscacides
• metaldehyde
• baysilon
• Acetaldehyde

Topics

• Acetaldehyde (analogs & derivatives, blood, poisoning)
• Dimethylpolysiloxanes
• Drug Stability
• Female
• Gas Chromatography-Mass Spectrometry (methods)
• Humans
• Linear Models
• Molluscacides (blood, poisoning)
• Reproducibility of Results
• Sensitivity and Specificity
• Solid Phase Microextraction (methods)
• Suicide, Attempted
• Young Adult