[Arsenic trioxide induces socs-1 gene demethylation in myeloma cell lines].
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| Abstract |
The aim of this study was to explore the effect of arsenic trioxide (As(2)O(3)) on the methylation status of socs-1 gene in multiple myeloma cell lines U266, RPMI8226. The cell viability was assayed by MTT method. The methylation status of socs-1 gene was detected by methylation specific PCR. The expression of socs-1 gene mRNA was determined with real-time PCR. The cell apoptosis was analyzed by flow cytometry. The results indicated that hypermethylation of CpG island of socs-1 gene was observed without expression of socs-1 in myeloma cell lines U266, RPMI8226. The expression of socs-1 gene mRNA in each myeloma cell line increased significantly after exposure to As(2)O(3) for 72 hours as compared with the cell lines of wild type (p < 0.05). And cell proliferation was significantly inhibited, both early apoptosis and later apoptosis ratios increased in dose-dependent manner. It is concluded that As(2)O(3) may induce socs-1 demethylation and up-regulate the expression of the gene. This study provides a new thought and direction for exploring possible mechanism of cell apoptosis induced by As(2)O(3) and multiple myeloma treatment by As(2)O(3).
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| Authors | Ming-Ming Wang, Qi Zhu, Zhi-Hong Ren, Li-Fang Zou, Hong-Ju Dou, Jun-Pei Hu |
| Journal | Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 16 Issue 5 Pg. 1064-8 (Oct 2008) ISSN: 1009-2137 [Print] China |
| PMID | 18928596 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Chemical References |
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