Abstract | PURPOSE:
Neuroblastomas frequently show expression of the epidermal growth factor receptor (EGFR) and may therefore be susceptible to EGFR-targeted therapies. Here, EGFR expression and functionality was investigated in parental chemosensitive neuroblastoma cell lines (UKF-NB-3, IMR-32, NLF, SH-SY5Y) and their cisplatin-resistant sublines (UKF-NB-3(r)CDDP(1000), IMR-32(r)CDDP(1000), NLF(r)CDDP(1000), and SH-SY5Y(r)CDDP(500)). Moreover, the EGFR antibody cetuximab, the EGFR tyrosine kinase inhibitor Tyrphostin B46, and recombinant EGFR- targeted toxins were investigated for their influence on the viability and growth of neuroblastoma cells. EXPERIMENTAL DESIGN: RESULTS: CONCLUSIONS: EGFR-targeted cytotoxic reagents such as scFv(14E1)-ETA and TGF-alpha-ETA represent promising candidates for further development as antineuroblastoma agents, especially in combination with cisplatin.
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Authors | Martin Michaelis, Jennifer Bliss, Sonja C Arnold, Nora Hinsch, Florian Rothweiler, Hedwig E Deubzer, Olaf Witt, Klaus Langer, Hans W Doerr, Winfried S Wels, Jindrich Cinatl Jr |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 14
Issue 20
Pg. 6531-7
(Oct 15 2008)
ISSN: 1078-0432 [Print] United States |
PMID | 18927293
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Monoclonal
- Antibodies, Monoclonal, Humanized
- Antineoplastic Agents
- Bacterial Toxins
- Exotoxins
- Protein Kinase Inhibitors
- Recombinant Fusion Proteins
- Transforming Growth Factor alpha
- Tyrphostins
- Virulence Factors
- alpha-cyano-(3,4-dihydroxy)-N-(3-phenylpropyl)cinnamide
- ADP Ribose Transferases
- Poly(ADP-ribose) Polymerases
- Pseudomonas aeruginosa exotoxin A
- ErbB Receptors
- Caspase 3
- Cetuximab
- Cisplatin
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Topics |
- ADP Ribose Transferases
(therapeutic use)
- Antibodies, Monoclonal
(adverse effects)
- Antibodies, Monoclonal, Humanized
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects)
- Bacterial Toxins
(therapeutic use)
- Blotting, Western
- Caspase 3
(metabolism)
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects)
- Cetuximab
- Cisplatin
(pharmacology)
- Drug Resistance, Neoplasm
- ErbB Receptors
(antagonists & inhibitors, genetics, metabolism)
- Exotoxins
(therapeutic use)
- Flow Cytometry
- Humans
- Neuroblastoma
(drug therapy, metabolism, pathology)
- Poly(ADP-ribose) Polymerases
(metabolism)
- Protein Kinase Inhibitors
(adverse effects)
- Recombinant Fusion Proteins
(therapeutic use)
- Transforming Growth Factor alpha
(administration & dosage, genetics)
- Tumor Cells, Cultured
- Tyrphostins
(adverse effects)
- Virulence Factors
(therapeutic use)
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