Abstract | PURPOSE: This study aimed to determine the effect of melatonin in preventing ischemia-reperfusion (I-R) injury-induced tissue damage and on spermatogenesis after experimental testicular torsion (TT). METHODS: Forty peripubertal rats were divided into 4 groups each containing 10 rats: control (C), sham (S), torsion plus detorsion (TD), and torsion plus melatonin (M). The left testes were rotated 720 degrees for 6 hours and detorsed for 6 hours thereafter. Serum inhibin B (IB) levels were measured in blood samples taken from all groups. Left orchiectomies were performed to determine the tissue levels of Johnsen's scores (JS) and malondialdehyde (MDA). RESULTS: Serum IB levels in the S and TD groups were significantly lower compared with that in the C group, whereas they were higher in the M group compared with the TD group. The MDA levels were significantly lower in the C, S, and, M groups compared with the TD group. Johnsen's scores were significantly higher in the C, S, and M groups compared with the TD group. CONCLUSIONS: Our results suggested that melatonin is a potent antioxidant agent in preventing testicular I-R injury, as shown by increased IB levels and JS.
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Authors | Müslim Yurtçu, Adnan Abasiyanik, Mustafa Cihat Avunduk, Sabahattin Muhtaroğlu |
Journal | Journal of pediatric surgery
(J Pediatr Surg)
Vol. 43
Issue 10
Pg. 1873-8
(Oct 2008)
ISSN: 1531-5037 [Electronic] United States |
PMID | 18926224
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antioxidants
- Biomarkers
- Free Radical Scavengers
- inhibin B
- Malondialdehyde
- Inhibins
- Glutathione Peroxidase
- Melatonin
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Topics |
- Animals
- Antioxidants
(therapeutic use)
- Biomarkers
- Drug Evaluation, Preclinical
- Free Radical Scavengers
(therapeutic use)
- Glutathione Peroxidase
(metabolism)
- Inhibins
(blood)
- Male
- Malondialdehyde
(analysis)
- Melatonin
(therapeutic use)
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(blood, etiology, pathology, prevention & control)
- Rotation
- Severity of Illness Index
- Sperm Count
- Spermatic Cord Torsion
(pathology, surgery)
- Spermatogenesis
(drug effects)
- Testis
(blood supply, chemistry, pathology)
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