Effect of artificial oxygen carrier with chemotherapy on tumor hypoxia and neovascularization.

Abstract

UNLABELLED:

In addition to transfusion alternatives, artificial oxygen carriers are a benefit in ischemia disorders. This study aimed at evaluating the possible effects of PEG-conjugated hemoglobin (PEG-Hb) plus cisplatin on tumor hypoxia and neovasculature.

METHODS:

HeLa cells were injected into submucosa of golden hamster cheek pouch to build tumor model. Animals were randomly assigned to 4 groups (n=10) and treated respectively: group 1, saline; group 2, cisplatin (5mg/kg); group 3, cisplatin (5mg/kg) plus PEG-Hb (0.3g/kg); group 4, cisplatin (5mg/kg) plus PEG-Hb (0.6g/kg). Tumor neovascularization morphological variation and tissue hypoxia were detected by intravital microscopy and immunostaining, respectively.

RESULTS:

Microvessel tortuosity and area capillary density in peritumoral areas were notably depressed in group 4 compared with group 2 (p<0.05). Hypoxia markers pimonidazole and HIF-1alpha expression were decreased significantly in group 4.

CONCLUSION:

PEG-Hb in high concentration can notably improve tumor tissue oxygenation and normalize neovasculature; it may be a potential adjuvant to chemotherapy in cancer.

• Authors: Jianqun Han, Minghua Yu, Min Dai, Peilin Cui, Hongwei Li, Jian Zhang, Qian Liu, Ruijuan Xiu
• Journal: Artificial cells, blood substitutes, and immobilization biotechnology (Artif Cells Blood Substit Immobil Biotechnol) Vol. 36 Issue 5 Pg. 431-8 ( 2008) ISSN: 1532-4184 [Electronic] England
• PMID: 18925466 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HIF1A protein, human
• Hemoglobins
• Hypoxia-Inducible Factor 1, alpha Subunit
• Nitroimidazoles
• Polyethylene Glycols
• pimonidazole
• Cisplatin
• Oxygen

Topics

• Animals
• Antineoplastic Combined Chemotherapy Protocols (therapeutic use)
• Chemotherapy, Adjuvant
• Cisplatin (administration & dosage)
• Cricetinae
• HeLa Cells
• Hemoglobins
• Humans
• Hypoxia (drug therapy)
• Hypoxia-Inducible Factor 1, alpha Subunit (administration & dosage)
• Mesocricetus
• Microvessels (pathology)
• Neoplasms, Experimental (blood supply, pathology, physiopathology)
• Neovascularization, Pathologic (drug therapy)
• Nitroimidazoles (administration & dosage)
• Oxygen (metabolism)
• Oxygen Inhalation Therapy (methods)
• Polyethylene Glycols
• Tumor Burden (drug effects)