Abstract | UNLABELLED: In addition to transfusion alternatives, artificial oxygen carriers are a benefit in ischemia disorders. This study aimed at evaluating the possible effects of PEG-conjugated hemoglobin ( PEG-Hb) plus cisplatin on tumor hypoxia and neovasculature. METHODS: HeLa cells were injected into submucosa of golden hamster cheek pouch to build tumor model. Animals were randomly assigned to 4 groups (n=10) and treated respectively: group 1, saline; group 2, cisplatin (5mg/kg); group 3, cisplatin (5mg/kg) plus PEG-Hb (0.3g/kg); group 4, cisplatin (5mg/kg) plus PEG-Hb (0.6g/kg). Tumor neovascularization morphological variation and tissue hypoxia were detected by intravital microscopy and immunostaining, respectively. RESULTS: Microvessel tortuosity and area capillary density in peritumoral areas were notably depressed in group 4 compared with group 2 (p<0.05). Hypoxia markers pimonidazole and HIF-1alpha expression were decreased significantly in group 4. CONCLUSION:
PEG-Hb in high concentration can notably improve tumor tissue oxygenation and normalize neovasculature; it may be a potential adjuvant to chemotherapy in cancer.
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Authors | Jianqun Han, Minghua Yu, Min Dai, Peilin Cui, Hongwei Li, Jian Zhang, Qian Liu, Ruijuan Xiu |
Journal | Artificial cells, blood substitutes, and immobilization biotechnology
(Artif Cells Blood Substit Immobil Biotechnol)
Vol. 36
Issue 5
Pg. 431-8
( 2008)
ISSN: 1532-4184 [Electronic] England |
PMID | 18925466
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- HIF1A protein, human
- Hemoglobins
- Hypoxia-Inducible Factor 1, alpha Subunit
- Nitroimidazoles
- Polyethylene Glycols
- pimonidazole
- Cisplatin
- Oxygen
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Topics |
- Animals
- Antineoplastic Combined Chemotherapy Protocols
(therapeutic use)
- Chemotherapy, Adjuvant
- Cisplatin
(administration & dosage)
- Cricetinae
- HeLa Cells
- Hemoglobins
- Humans
- Hypoxia
(drug therapy)
- Hypoxia-Inducible Factor 1, alpha Subunit
(administration & dosage)
- Mesocricetus
- Microvessels
(pathology)
- Neoplasms, Experimental
(blood supply, pathology, physiopathology)
- Neovascularization, Pathologic
(drug therapy)
- Nitroimidazoles
(administration & dosage)
- Oxygen
(metabolism)
- Oxygen Inhalation Therapy
(methods)
- Polyethylene Glycols
- Tumor Burden
(drug effects)
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