Molecular cloning and characterization of a 2C-methyl-D: -erythritol 2,4-cyclodiphosphate synthase gene from Cephalotaxus harringtonia.

Abstract

The full-length MECPS cDNA sequence (designated as Chmecps, GenBank Accession No.: DQ415658) was isolated by rapid amplification of cDNA ends (RACE) for the first time from Cephalotaxus harringtonia. The full-length cDNA of Chmecps was 1,146 bp containing a 753 bp open reading frame (ORF) encoding a polypeptide of 250 amino acids with a calculated mass of 26.67 kDa and an isoelectric point of 9.35. Comparative and bioinformatics analyses revealed that ChMECPS showed extensive homology with MECPSs from other plant species. Phylogenetic analysis indicated ChMECPS was more ancient than other plant MECPSs. Southern hybridization analysis of the genomic DNA showed that Chmecps was a single copy gene. Tissue expression pattern analysis revealed that ChMECPS expressed strongly in root and leaf, weakly in stem.

• Authors: Junfeng Chen, Ying Xiao, Peng Di, Xiaojing Yu, Wansheng Chen, Lei Zhang
• Journal: Molecular biology reports (Mol Biol Rep) Vol. 36 Issue 7 Pg. 1749-56 (Sep 2009) ISSN: 1573-4978 [Electronic] Netherlands
• PMID: 18923920 (Publication Type: Journal Article)

Chemical References

• DNA, Complementary
• Phosphorus-Oxygen Lyases

Topics

• Amino Acid Sequence
• Base Sequence
• Blotting, Southern
• Cephalotaxus (enzymology, genetics)
• Cloning, Molecular
• Computational Biology
• DNA, Complementary (genetics)
• Evolution, Molecular
• Gene Expression Profiling
• Gene Expression Regulation, Enzymologic
• Gene Expression Regulation, Plant
• Genes, Plant
• Molecular Sequence Data
• Phosphorus-Oxygen Lyases (chemistry, genetics, metabolism)
• Phylogeny
• Protein Structure, Secondary
• Reverse Transcriptase Polymerase Chain Reaction
• Sequence Alignment
• Sequence Analysis, DNA