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A sindbis virus replicon-based DNA vaccine encoding the rabies virus glycoprotein elicits immune responses and complete protection in mice from lethal challenge.

Abstract
A sindbis virus replicon-based DNA vaccine encoding rabies virus glycoprotein (G) was developed by subcloning rabies G gene into a sindbis virus replicon-based vaccine vector (pAlpha). The self-amplification of RNA transcripts and translation efficiency of rabies G was analyzed in pAlpha-Rab-G-transfected mammalian cells using RT-PCR, SDS-PAGE and Western blot analysis. The transfected cells also showed induction of apoptosis which is an important event in the enhancement of immune responses. Further, immune responses induced with replicon-based rabies DNA vaccine (pAlpha-Rab-G) was compared with conventional rabies DNA vaccine and commercial cell culture vaccine (Rabipur) in intramuscularly injected mice. The mice immunized with replicon-based rabies DNA vaccine induced humoral and cell mediated immune responses better than conventional rabies DNA vaccine however, comparable to Rabipur vaccine. On challenge with rabies virus CVS strain, replicon-based rabies DNA vaccine conferred complete protection similar to Rabipur. These results demonstrate that replicon-based rabies DNA vaccine is effective in inducing both humoral and cellular immune responses and can be considered as effective vaccine against rabies.
AuthorsSonal Saxena, Shyam S Dahiya, Arvind A Sonwane, Chhabi Lal Patel, Mohini Saini, A Rai, Praveen K Gupta
JournalVaccine (Vaccine) Vol. 26 Issue 51 Pg. 6592-601 (Dec 02 2008) ISSN: 0264-410X [Print] Netherlands
PMID18848857 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Viral
  • Antigens, Viral
  • Cytokines
  • Glycoproteins
  • Immunoglobulin G
  • Rabies Vaccines
  • Vaccines, DNA
  • Viral Envelope Proteins
  • glycoprotein G, Rabies virus
Topics
  • Animals
  • Antibodies, Viral (blood)
  • Antigens, Viral (immunology)
  • Apoptosis
  • Cell Proliferation
  • Cells, Cultured
  • Cricetinae
  • Cytokines (immunology)
  • Glycoproteins (immunology)
  • Immunization, Secondary
  • Immunoglobulin G (blood)
  • Mice
  • Rabies (prevention & control)
  • Rabies Vaccines (genetics, immunology)
  • Rabies virus (immunology)
  • Replicon
  • Sindbis Virus (immunology)
  • Vaccines, DNA (immunology)
  • Viral Envelope Proteins (immunology)

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