Abstract |
A sindbis virus replicon-based DNA vaccine encoding rabies virus glycoprotein (G) was developed by subcloning rabies G gene into a sindbis virus replicon-based vaccine vector (pAlpha). The self-amplification of RNA transcripts and translation efficiency of rabies G was analyzed in pAlpha-Rab-G-transfected mammalian cells using RT-PCR, SDS-PAGE and Western blot analysis. The transfected cells also showed induction of apoptosis which is an important event in the enhancement of immune responses. Further, immune responses induced with replicon-based rabies DNA vaccine (pAlpha-Rab-G) was compared with conventional rabies DNA vaccine and commercial cell culture vaccine (Rabipur) in intramuscularly injected mice. The mice immunized with replicon-based rabies DNA vaccine induced humoral and cell mediated immune responses better than conventional rabies DNA vaccine however, comparable to Rabipur vaccine. On challenge with rabies virus CVS strain, replicon-based rabies DNA vaccine conferred complete protection similar to Rabipur. These results demonstrate that replicon-based rabies DNA vaccine is effective in inducing both humoral and cellular immune responses and can be considered as effective vaccine against rabies.
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Authors | Sonal Saxena, Shyam S Dahiya, Arvind A Sonwane, Chhabi Lal Patel, Mohini Saini, A Rai, Praveen K Gupta |
Journal | Vaccine
(Vaccine)
Vol. 26
Issue 51
Pg. 6592-601
(Dec 02 2008)
ISSN: 0264-410X [Print] Netherlands |
PMID | 18848857
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- Antigens, Viral
- Cytokines
- Glycoproteins
- Immunoglobulin G
- Rabies Vaccines
- Vaccines, DNA
- Viral Envelope Proteins
- glycoprotein G, Rabies virus
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Topics |
- Animals
- Antibodies, Viral
(blood)
- Antigens, Viral
(immunology)
- Apoptosis
- Cell Proliferation
- Cells, Cultured
- Cricetinae
- Cytokines
(immunology)
- Glycoproteins
(immunology)
- Immunization, Secondary
- Immunoglobulin G
(blood)
- Mice
- Rabies
(prevention & control)
- Rabies Vaccines
(genetics, immunology)
- Rabies virus
(immunology)
- Replicon
- Sindbis Virus
(immunology)
- Vaccines, DNA
(immunology)
- Viral Envelope Proteins
(immunology)
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