[Studies of betuionic acid on cell cycle and related protein expressions on mice of bearing H22 tumor cells].

Abstract

OBJECTIVE:

To study the betulonic acid on the cell cycle and related protein expressions on mice of bearing H22 tumor cells.

METHOD:

Flow cytometray was used to observe the changes of betulonic acid on the cell cycle and P53 of H22 tumor cells. Immunohistochemistry was determined the espressions of PI3K and AKT.

RESULT:

Increasing the doses of betulonic acid, the number of H22 cells in S phase and G2 phase was increasing gradually, it can speculate that when the betulonic acid act on cells, the cells were blocked in S and G2 phase and inhibited the protein expressions of PI3K and AKT.

CONCLUSION:

Betulonic acid may be up-regulate the activity of P53 and inhibite the expressions of PI3K and AKT, so that it inhibited the survival pathway of tumor cells.

• Authors: Xiu-juan Zhang, Lei Han, Yu-bin Ji, Gui-zhen Fang
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 33 Issue 14 Pg. 1739-43 (Jul 2008) ISSN: 1001-5302 [Print] China
• PMID: 18841782 (Publication Type: English Abstract, Journal Article)

Chemical References

• Drugs, Chinese Herbal
• Tumor Suppressor Protein p53
• Phosphatidylinositol 3-Kinases
• Proto-Oncogene Proteins c-akt

Topics

• Animals
• Betula (chemistry)
• Cell Cycle (drug effects)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Drugs, Chinese Herbal (chemistry, pharmacology)
• Female
• Flow Cytometry
• G2 Phase (drug effects)
• Gene Expression Regulation (drug effects)
• Immunohistochemistry
• Male
• Mice
• Neoplasm Transplantation
• Phosphatidylinositol 3-Kinases (metabolism)
• Proto-Oncogene Proteins c-akt (metabolism)
• Tumor Suppressor Protein p53 (metabolism)