[Analysis of chemical constituents of anti-myocardial ischemia fraction of Corydalis yanhusuo].

Abstract

OBJECTIVE:

To establish the HPLC fingerprint of anti-myocardial ischemia fraction of Corydalis yanhusuo, and to investigate its chemical constituents by LC-MS-MS.

METHOD:

The separation was performed at 30 degrees C on a Dikma Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) by isocratic elution with acetonitrile-water (23:77) (containing 0.8% acetic acid and 0.2% triethylamine) as the mobile phase at a flow rate of 1.0 mL x min(-1), with UV detection at 335 nm.

RESULT:

A satisfactory fingerprint was obtained by HPLC method, 12 common peaks were selected as the fingerprint peaks, and 8 among them were identified according to the chromatographic behavior and mass spectroscopic data by comparison with those of authentic standard.

CONCLUSION:

This method is simple, sensitive and reliable, can be used to investigate the chemical constituents of anti-myocardial ischemia fraction of C. yanhusuo and control the quality.

• Authors: Xing-ye Cheng, Yue Shi
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 33 Issue 14 Pg. 1717-9 (Jul 2008) ISSN: 1001-5302 [Print] China
• PMID: 18841776 (Publication Type: English Abstract, Journal Article)

Chemical References

• Drugs, Chinese Herbal

Topics

• Chromatography, High Pressure Liquid
• Corydalis (chemistry)
• Drugs, Chinese Herbal (chemistry)
• Tandem Mass Spectrometry