Abstract | OBJECTIVE: To establish the HPLC fingerprint of anti-myocardial ischemia fraction of Corydalis yanhusuo, and to investigate its chemical constituents by LC-MS-MS. METHOD: The separation was performed at 30 degrees C on a Dikma Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) by isocratic elution with acetonitrile-water (23:77) (containing 0.8% acetic acid and 0.2% triethylamine) as the mobile phase at a flow rate of 1.0 mL x min(-1), with UV detection at 335 nm. RESULT: A satisfactory fingerprint was obtained by HPLC method, 12 common peaks were selected as the fingerprint peaks, and 8 among them were identified according to the chromatographic behavior and mass spectroscopic data by comparison with those of authentic standard. CONCLUSION: This method is simple, sensitive and reliable, can be used to investigate the chemical constituents of anti-myocardial ischemia fraction of C. yanhusuo and control the quality.
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Authors | Xing-ye Cheng, Yue Shi |
Journal | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
(Zhongguo Zhong Yao Za Zhi)
Vol. 33
Issue 14
Pg. 1717-9
(Jul 2008)
ISSN: 1001-5302 [Print] China |
PMID | 18841776
(Publication Type: English Abstract, Journal Article)
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Chemical References |
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Topics |
- Chromatography, High Pressure Liquid
- Corydalis
(chemistry)
- Drugs, Chinese Herbal
(chemistry)
- Tandem Mass Spectrometry
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