[Study of gastric carcinoma and PCNA and c-met gene abnormality].

Abstract

OBJECTIVE:

To explore the changed regularity of proliferating cell nuclear antigen (PCNA) and c-met gene abnormality in the oncogenic pathway of gastric carcinoma.

METHODS:

Semi-quantitative PCR was used to detect the amplification of c-met gene in 58 gastric carcinoma tissues and corresponding para-carcinoma tissues. Immunohistochemical staining was used to detect the expression and over expression of c-met and PCNA protein.

RESULTS:

The amplification of c-met was detected in 58 cases was significant statistic difference on the amplification of c-met oncogene between the two groups (t = 9.455, P < 0.01). The positive rate of c-met protein in gastric carcinoma tissues was 67.24% (39/58), which was negative in the corresponding para-carcinoma tissues. There was significant difference between the two groups (chi2 = 58.75, P < 0.001). PCNA LI of 58 gastric carcinoma tissues and the corresponding adjacent tissues were 49.3768 +/- 12.1832 and 14.8390 +/- 7.1847, respectively. The PCNA LI had a significant difference between the two groups( t = 8.805, P < 0.01) . The amplification and overexpression of c-met gene in gastric carcinoma tissues has no relationship with the tumor's size, location, lymph node metastasis, differentiation degree and deeper invasion. The amplification of c-met oncogene was related with the oncogenesis of gastric carcinoma.

CONCLUSION:

The abnormal expression of c-met and PCNA protein was related with the oncogenesis of gastric carcinoma.

• Authors: Jing Ji, Peng Zhao, Baohua Huang
• Journal: Wei sheng yan jiu = Journal of hygiene research (Wei Sheng Yan Jiu) Vol. 37 Issue 4 Pg. 479-82 (Jul 2008) ISSN: 1000-8020 [Print] China
• PMID: 18839537 (Publication Type: English Abstract, Journal Article)

Chemical References

• Proliferating Cell Nuclear Antigen
• Proto-Oncogene Proteins c-met

Topics

• Female
• Gene Amplification (genetics)
• Humans
• Male
• Middle Aged
• Polymerase Chain Reaction (methods)
• Proliferating Cell Nuclear Antigen (genetics, metabolism)
• Proto-Oncogene Proteins c-met (genetics, metabolism)
• Stomach Neoplasms (genetics, metabolism, pathology)