Protective effect of ilomastat on trinitrobenzenesulfonic acid-induced ulcerative colitis in rats.

Abstract

AIM:

To evaluate the protective effects of ilomastat, an exogenous matrix metalloproteinase (MMP) inhibitor, on trinitrobenzenesulfonic acid (TNB)-induced ulcerative colitis (UC) in rats.

METHODS:

Male SD rats were randomly divided into model group, protective groups A and B, and normal control group. Rats in the model group received only intra-colonic TNB. Rats in the protective groups A and B received intra-peritoneal ilomastat of 10 mg/kg and 20 mg/kg, respectively, beside TNB. Rats in the normal control group received only intra-colonic normal saline. After 3 wk, segments of colon were obtained. RT-PCR and immunohistochemistry were used to examine the expression of MMP-1 and TIMP-1. Hematoxylin-eosin (HE) staining was used for pathological study.

RESULTS:

The model of UC was successfully induced in rats. Inflammation of colonic mucosa greatly improved in protective groups A and B. Expression of MMP-1 and TIMP-1 in the model group, protective groups A and B was significantly higher than that in the normal control group (P < 0.0001) with MMP-1 expression increased more significantly than TIMP-1 expression. Expression of MMP-1 in protective groups A and B was significantly lower than that in the model group (P < 0.0001) . Expression of MMP-1 in protective group B was significantly lower than that in protective group A (P < 0.0001).

CONCLUSION:

Ilomastat improves TNB-induced UC in rats by inhibiting the MMP-1 activity.

• Authors: Ying-De Wang, Wei Wang
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 14 Issue 37 Pg. 5683-8 (Oct 07 2008) ISSN: 1007-9327 [Print] United States
• PMID: 18837084 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Gastrointestinal Agents
• Hydroxamic Acids
• Indoles
• Matrix Metalloproteinase Inhibitors
• Protease Inhibitors
• RNA, Messenger
• Tissue Inhibitor of Metalloproteinase-1
• Trinitrobenzenesulfonic Acid
• Matrix Metalloproteinase 1
• ilomastat

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Colitis, Ulcerative (chemically induced, enzymology, prevention & control)
• Colon (drug effects, enzymology, pathology)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Gastrointestinal Agents (pharmacology)
• Hydroxamic Acids
• Immunohistochemistry
• Indoles (pharmacology)
• Male
• Matrix Metalloproteinase 1 (genetics, metabolism)
• Matrix Metalloproteinase Inhibitors
• Protease Inhibitors (pharmacology)
• RNA, Messenger (metabolism)
• Rats
• Rats, Sprague-Dawley
• Reverse Transcriptase Polymerase Chain Reaction
• Tissue Inhibitor of Metalloproteinase-1 (metabolism)
• Trinitrobenzenesulfonic Acid