Electrical detection of VEGFs for cancer diagnoses using anti-vascular endotherial growth factor aptamer-modified Si nanowire FETs.

Abstract

We report the real-time, label-free and electrical detection of vascular endotherial growth factor (VEGF) for cancer diagnosis using anti-VEGF aptamer-modified Si nanowire field-effect transistors (SiNW-FETs). Specifically, the high quality single-crystalline SiNWs of both n-type and p-type characters were surface modified with the covalent immobilization of anti-VEGF aptamers, and they were turned into SiNW-FET biosensors for the VEGF detection. We show that the VEGF molecules consistently act on the gate dielectrics of both n-type and p-type SiNW-FETs as electrically positive point-charges; their recognition to anti-VEGF aptamers depletes (accumulates) the charge carriers in the p-type (n-type) SiNW-FETs and thus decreases (increases) the detection currents. The detection limit for VEGFs in this study was determined as 1.04nM and 104pM for the cases of n-type and p-type SiNW-FETs, respectively.

• Authors: Hyun-Seung Lee, Ki Su Kim, Cheol-Joo Kim, Sei Kwang Hahn, Moon-Ho Jo
• Journal: Biosensors & bioelectronics (Biosens Bioelectron) Vol. 24 Issue 6 Pg. 1801-5 (Feb 15 2009) ISSN: 1873-4235 [Electronic] England
• PMID: 18835770 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Aptamers, Peptide
• Biomarkers, Tumor
• Neoplasm Proteins
• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• Silicon

Topics

• Aptamers, Peptide (chemistry)
• Biomarkers, Tumor (analysis)
• Biosensing Techniques (instrumentation)
• Electrochemistry (instrumentation)
• Equipment Design
• Equipment Failure Analysis
• Immunoassay (instrumentation)
• Nanotechnology (instrumentation, methods)
• Nanotubes (chemistry, ultrastructure)
• Neoplasm Proteins (analysis)
• Neoplasms (diagnosis, metabolism)
• Reproducibility of Results
• Sensitivity and Specificity
• Silicon (chemistry)
• Transistors, Electronic
• Vascular Endothelial Growth Factor A (analysis, chemistry)