| Abstract | We have developed an experimental recombinant vesicular stomatitis virus (VSV) vectored plague vaccine expressing a secreted form of Yersinia pestis low calcium response protein V (LcrV) from the first position of the VSV genome. This vector, given intramuscularly in a single dose, induced high-level antibody titers to LcrV and gave 90-100% protection against pneumonic plague challenge in mice. This single-dose protection was significantly better than that generated by VSV expressing the non-secreted LcrV protein. Increased protection correlated with increased anti-LcrV antibody and a bias toward IgG2a and away from IgG1 isotypes. We also found that the depletion of CD4+ cells, but not CD8+ cells, at the time of challenge resulted in reduced vaccine protection, indicating a role for cellular immunity in protection. |
| Authors | Anasuya Chattopadhyay, Steven Park, Guillaume Delmas, Rema Suresh, Svetlana Senina, David S Perlin, John K Rose
(Affiliation: Department of Pathology, Yale University School of Medicine, 310 Cedar Street LH 315, New Haven, CT 06510, USA.)
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| Journal | Vaccine
(Vaccine)
Vol. 26
Issue 50
Pg. 6329-37
(Nov 25 2008)
ISSN: 0264-410X Netherlands |
| PMID | 18832004
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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