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Novel process of fermenting black soybean [Glycine max (L.) Merrill] yogurt with dramatically reduced flatulence-causing oligosaccharides but enriched soy phytoalexins.

Abstract
Black soybeans [Glycine max (L.) Merrill] were germinated under fungal stress with food grade R. oligosporus for 3 days and were homogenized and fermented with lactic acid bacteria (LAB) to produce soy yogurt. Fungal stress led to the generation of oxylipins [oxooctadecadienoic acids (KODES) isomers and their respective glyceryl esters] and glyceollins--a type of phytoalexins unique to soybeans. In soy yogurt, the concentrations of total KODES and total glyceollins were 0.678 mg/g (dry matter) and 0.953 mg/g, respectively. The concentrations of other isoflavones (mainly genistein and daidzein and their derivatives) in soy yogurt remained largely unchanged after the processes compared with the control soy yogurt. Germination of black soybean under fungal stress for 3 days was sufficient to reduce stachyose and raffinose (which cause flatulence) by 92 and 80%, respectively. With a pH value of 4.42, a lactic acid content of 0.262%, and a maximum viable cell count of 2.1 x 10 (8) CFU/mL in the final soy yogurt, soy milk from germinated soybeans under fungal stress was concluded to be a suitable medium for yogurt-making. The resulting soy yogurt had significantly altered micronutrient profiles with significantly reduced oligosaccharides and enriched glyceollins.
AuthorsShengbao Feng, Chin Lee Saw, Yuan Kun Lee, Dejian Huang
JournalJournal of agricultural and food chemistry (J Agric Food Chem) Vol. 56 Issue 21 Pg. 10078-84 (Nov 12 2008) ISSN: 1520-5118 [Electronic] United States
PMID18831591 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Oligosaccharides
  • Pterocarpans
  • Sesquiterpenes
  • Terpenes
  • Lactic Acid
  • glyceollin
  • Phytoalexins
Topics
  • Bacteria (metabolism)
  • Fermentation
  • Flatulence (microbiology)
  • Food Handling (methods)
  • Lactic Acid (metabolism)
  • Oligosaccharides (metabolism)
  • Pterocarpans (analysis)
  • Rhizopus (metabolism)
  • Sesquiterpenes
  • Soybeans (chemistry, metabolism)
  • Terpenes (metabolism)
  • Yogurt (analysis, microbiology)
  • Phytoalexins

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