Abstract | PURPOSE: METHODS: HRCECs were transfected with an ALK1 gene-encoding plasmid or a pSIREN-ALK1 RNAi vector and stimulated with VEGF. The mRNA and protein expression levels of ALK1, occludin, ANG2, and ALK5 were evaluated by real-time PCR and/or Western blot analysis. Microscopy techniques and flow cytometry were used to assess the effects of enhanced levels of ALK1 on migration and proliferation and the formation of tubelike structures of HRCECs. RESULTS: The level of ALK1 in ALK1-transfected cells was significantly increased compared with that in control cells. ALK1-transfected cells exhibited increased expression of occludin and decreased expression of ANG2 and ALK5, compared with expression in the control cells. HRCECs transfected with pSIREN-ALK1 RNAi exhibited decreased expression of ALK1 and occludin and increased expression of ANG2 and ALK5 compared with the control cells. Transfection with ALK1 affected the migration and proliferation of VEGF-stimulated HRCECs. ALK1 also inhibited the formation of endothelial tubelike structures, but did allow the formation of entire vessel structures. CONCLUSIONS:
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Authors | Bin Li, Wei Yin, Xun Hong, Yu Shi, Hong-Sheng Wang, Shao-Fen Lin, Shi-Bo Tang |
Journal | Investigative ophthalmology & visual science
(Invest Ophthalmol Vis Sci)
Vol. 49
Issue 10
Pg. 4553-60
(Oct 2008)
ISSN: 1552-5783 [Electronic] United States |
PMID | 18829861
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Angiopoietin-2
- Membrane Proteins
- OCLN protein, human
- Occludin
- RNA, Messenger
- Receptors, Transforming Growth Factor beta
- Vascular Endothelial Growth Factor A
- Protein Serine-Threonine Kinases
- ACVRL1 protein, human
- Activin Receptors, Type II
- Receptor, Transforming Growth Factor-beta Type I
- TGFBR1 protein, human
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Topics |
- Activin Receptors, Type II
(genetics, metabolism)
- Adult
- Angiopoietin-2
(genetics)
- Blotting, Western
- Capillaries
- Cell Movement
- Cell Proliferation
- Cells, Cultured
- Endothelium, Vascular
(drug effects, metabolism, pathology)
- Flow Cytometry
- Genetic Vectors
- Humans
- Membrane Proteins
(genetics)
- Occludin
- Plasmids
- Protein Serine-Threonine Kinases
(genetics)
- RNA, Messenger
(metabolism)
- Receptor, Transforming Growth Factor-beta Type I
- Receptors, Transforming Growth Factor beta
(genetics)
- Retinal Neovascularization
(metabolism, pathology, prevention & control)
- Retinal Vessels
(physiology)
- Reverse Transcriptase Polymerase Chain Reaction
- Transfection
- Vascular Endothelial Growth Factor A
(pharmacology)
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