Abstract | INTRODUCTION: MATERIALS AND METHODS: We analyzed the expression of pro-inflammatory IL-12p35, IL-12p40, IL-23p19, anti-inflammatory IL-10, antiapoptotic factor survivin, and transcription factors-RelA, c-Jun, and Foxp3 mRNA in patients' blood, colon carcinoma tissue, and in normal mucosal tissue by real-time polymerase chain reaction. The quantity determination of serum IL-12p40, IL-23, and IL-10 was performed by enzyme-linked immunosorbent assay. RESULTS: CONCLUSIONS: In conclusion, we show a characteristic gene expression profile combining markers associated with inhibition of anti- tumor immune response (Foxp3, IL-10), inhibition of apoptosis ( survivin), and induction of the cytokines with protumoral activity as IL-12p40 and IL-23p19 (IL-23) in the colorectal tumor tissue but not in peripheral blood of patients.
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Authors | Noyko Stanilov, Lyuba Miteva, Nikolay Mintchev, Spaska Stanilova |
Journal | International journal of colorectal disease
(Int J Colorectal Dis)
Vol. 24
Issue 2
Pg. 151-7
(Feb 2009)
ISSN: 1432-1262 [Electronic] Germany |
PMID | 18825388
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- BIRC5 protein, human
- FOXP3 protein, human
- Forkhead Transcription Factors
- Inhibitor of Apoptosis Proteins
- Interleukin-12 Subunit p35
- Interleukin-12 Subunit p40
- Interleukin-23 Subunit p19
- Microtubule-Associated Proteins
- RNA, Messenger
- Survivin
- Transcription Factors
- Interleukin-10
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Topics |
- Colorectal Neoplasms
(blood, genetics, surgery)
- Forkhead Transcription Factors
(genetics, metabolism)
- Gene Expression Regulation, Neoplastic
- Humans
- Inhibitor of Apoptosis Proteins
- Interleukin-10
(blood, genetics)
- Interleukin-12 Subunit p35
(genetics, metabolism)
- Interleukin-12 Subunit p40
(blood, genetics)
- Interleukin-23 Subunit p19
(blood, genetics)
- Intestinal Mucosa
(metabolism, pathology)
- Microtubule-Associated Proteins
(genetics, metabolism)
- RNA, Messenger
(blood, genetics)
- Survivin
- Transcription Factors
(genetics, metabolism)
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