HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

High expression of Foxp3, IL-23p19 and survivin mRNA in colorectal carcinoma.

AbstractINTRODUCTION:
Cytokines have been suggested to both modulate anti-tumor responses and promote tumor growth.
MATERIALS AND METHODS:
We analyzed the expression of pro-inflammatory IL-12p35, IL-12p40, IL-23p19, anti-inflammatory IL-10, antiapoptotic factor survivin, and transcription factors-RelA, c-Jun, and Foxp3 mRNA in patients' blood, colon carcinoma tissue, and in normal mucosal tissue by real-time polymerase chain reaction. The quantity determination of serum IL-12p40, IL-23, and IL-10 was performed by enzyme-linked immunosorbent assay.
RESULTS:
We observed significantly higher levels in patients for all three analyzed cytokines, with IL-23 concentration change being the highest. We detected the greatest upregulation of IL-23p19, Foxp3 and survivin mRNA in colorectal carcinomas than normal mucosa. A statistically significant upregulation of IL-12p40, IL-10, and c-Jun mRNA but not for IL-12p35 and RelA mRNA in tumor tissue comparing to normal tissue was also established.
CONCLUSIONS:
In conclusion, we show a characteristic gene expression profile combining markers associated with inhibition of anti-tumor immune response (Foxp3, IL-10), inhibition of apoptosis (survivin), and induction of the cytokines with protumoral activity as IL-12p40 and IL-23p19 (IL-23) in the colorectal tumor tissue but not in peripheral blood of patients.
AuthorsNoyko Stanilov, Lyuba Miteva, Nikolay Mintchev, Spaska Stanilova
JournalInternational journal of colorectal disease (Int J Colorectal Dis) Vol. 24 Issue 2 Pg. 151-7 (Feb 2009) ISSN: 1432-1262 [Electronic] Germany
PMID18825388 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • BIRC5 protein, human
  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • Inhibitor of Apoptosis Proteins
  • Interleukin-12 Subunit p35
  • Interleukin-12 Subunit p40
  • Interleukin-23 Subunit p19
  • Microtubule-Associated Proteins
  • RNA, Messenger
  • Survivin
  • Transcription Factors
  • Interleukin-10
Topics
  • Colorectal Neoplasms (blood, genetics, surgery)
  • Forkhead Transcription Factors (genetics, metabolism)
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Inhibitor of Apoptosis Proteins
  • Interleukin-10 (blood, genetics)
  • Interleukin-12 Subunit p35 (genetics, metabolism)
  • Interleukin-12 Subunit p40 (blood, genetics)
  • Interleukin-23 Subunit p19 (blood, genetics)
  • Intestinal Mucosa (metabolism, pathology)
  • Microtubule-Associated Proteins (genetics, metabolism)
  • RNA, Messenger (blood, genetics)
  • Survivin
  • Transcription Factors (genetics, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: