Genetic and histological studies on the delayed systemic movement of Tobacco Mosaic Virus in Arabidopsis thaliana.

Abstract

BACKGROUND:

Viral infections and their spread throughout a plant require numerous interactions between the host and the virus. While new functions of viral proteins involved in these processes have been revealed, current knowledge of host factors involved in the spread of a viral infection is still insufficient. In Arabidopsis thaliana, different ecotypes present varying susceptibilities to Tobacco mosaic virus strain U1 (TMV-U1). The rate of TMV-U1 systemic movement is delayed in ecotype Col-0 when compared with other 13 ecotypes.We followed viral movement through vascular tissue in Col-0 plants by electronic microscopy studies. In addition, the delay in systemic movement of TMV-U1 was genetically studied.

RESULTS:

TMV-U1 reaches apical leaves only after 18 days post rosette inoculation (dpi) in Col-0, whereas it is detected at 9 dpi in the Uk-4 ecotype. Genetic crosses between Col-0 and Uk-4 ecotypes, followed by analysis of viral movement in F1 and F2 populations, revealed that this delayed movement correlates with a recessive, monogenic and nuclear locus. The use of selected polymorphic markers showed that this locus, denoted DSTM1 (Delayed Systemic Tobamovirus Movement 1), is positioned on the large arm of chromosome II. Electron microscopy studies following the virion's route in stems of Col-0 infected plants showed the presence of curved structures, instead of the typical rigid rods of TMV-U1. This was not observed in the case of TMV-U1 infection in Uk-4, where the observed virions have the typical rigid rod morphology.

CONCLUSION:

The presence of defectively assembled virions observed by electron microscopy in vascular tissue of Col-0 infected plants correlates with a recessive delayed systemic movement trait of TMV-U1 in this ecotype.

• Authors: Carolina Serrano, Javiera González-Cruz, Francisca Jauregui, Consuelo Medina, Pablo Mancilla, José Tomás Matus, Patricio Arce-Johnson
• Journal: BMC genetics (BMC Genet) Vol. 9 Pg. 59 (Sep 26 2008) ISSN: 1471-2156 [Electronic] England
• PMID: 18817581 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Plant Viral Movement Proteins

Topics

• Arabidopsis (cytology, genetics, virology)
• Chromosome Mapping
• Chromosomes, Plant
• Genes, Plant
• Host-Pathogen Interactions (genetics, physiology)
• Models, Biological
• Movement (physiology)
• Plant Leaves (ultrastructure, virology)
• Plant Viral Movement Proteins (genetics, physiology)
• Reaction Time (genetics, physiology)
• Time Factors
• Tobacco Mosaic Virus (genetics, physiology)
• Virus Latency (genetics, physiology)