| Abstract | In order to address whether galectin-3 in the sera and fine needle aspirates serve as a diagnostic marker distinguishing between benign and malignant thyroid nodules, we developed an enzyme-linked immunosorbent assay. We quantified galectin-3 in fine needle aspirates from a series of 118 patients with thyroid nodules and serum galectin-3 from another series of 46 patients, which were compared with final histology after thyroidectomy. Relative galectin-3 value (ng/mg), defined as galectin-3 concentration (ng/ml) divided by total protein concentration (mg/ml) in fine needle aspirates, was significantly higher in papillary carcinoma than in the other thyroid entities. There was no significant difference in serum galectin-3 level among patients with thyroid nodules and healthy individuals. Accordingly, relative galectin-3 value allows a definitive diagnosis of papillary carcinoma independent of cellular morphology, whereas serum galectin-3 does not serve as a marker for papillary carcinoma. |
| Authors | Hidenori Inohara, Tatsuya Segawa, Akira Miyauchi, Tadashi Yoshii, Susumu Nakahara, Avraham Raz, Masahiro Maeda, Eiji Miyoshi, Noriaki Kinoshita, Hiroshi Yoshida, Masashi Furukawa, Yukinori Takenaka, Yuki Takamura, Yasuhiro Ito, Naoyuki Taniguchi
(Affiliation: Department of Otolaryngology, Osaka University School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan. hinohara at ent.med.osaka-u.ac.jp)
|
| Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 376
Issue 3
Pg. 605-10
(Nov 21 2008)
ISSN: 1090-2104 United States |
| PMID | 18809382
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Antibodies, Monoclonal
- Galectin 3
- Tumor Markers, Biological
|
| Topics |
- Antibodies, Monoclonal
(immunology)
- Biopsy, Fine-Needle
- Carcinoma, Papillary
(diagnosis, pathology)
- Cytoplasm
(chemistry)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Galectin 3
(analysis, blood)
- Humans
- Immunochemistry
- Thyroid Nodule
(diagnosis, pathology)
- Tumor Markers, Biological
(analysis, blood)
|
