Abstract | BACKGROUND: AIM OF THE STUDY: METHODS:
Insulin sensitivity in L6 myotubes was assessed by 2-deoxy-D: -[(3)H]- glucose uptake. The phosphorylation of distal insulin signaling proteins Akt and the Akt substrate AS160 were determined by western blot. RESULTS: One hour treatment with 100 mU/ml GO decreased insulin-stimulated glucose uptake (P < 0.001). Pre-treatment with GT either partially (100 microM) or completely (200 microM) restored insulin-stimulated glucose uptake in cells after GO-induced insulin resistance. GO-induced oxidative stress did not impair insulin stimulated phosphorylation of Akt or AS160, but 200 microM GT increased insulin-stimulated phosphorylation of these key signaling proteins (P < 0.05). CONCLUSIONS: High-dose (200 microM) GT treatment ameliorated oxidative stress-induced insulin resistance in cultured rat L6 skeletal muscle cells.
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Authors | Indu Singh, Andrew L Carey, Nadine Watson, Mark A Febbraio, John A Hawley |
Journal | European journal of nutrition
(Eur J Nutr)
Vol. 47
Issue 7
Pg. 387-92
(Oct 2008)
ISSN: 1436-6215 [Electronic] Germany |
PMID | 18807106
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Blood Glucose
- Insulin
- Reactive Oxygen Species
- gamma-Tocopherol
- Glucose Oxidase
- Proto-Oncogene Proteins c-akt
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Topics |
- Analysis of Variance
- Animals
- Blood Glucose
(metabolism)
- Blotting, Western
- Cells, Cultured
- Glucose Oxidase
(toxicity)
- Humans
- Insulin
(metabolism)
- Insulin Resistance
- Muscle, Skeletal
(metabolism, physiology)
- Oxidative Stress
(drug effects)
- Phosphorylation
- Proto-Oncogene Proteins c-akt
(metabolism)
- Rats
- Reactive Oxygen Species
- gamma-Tocopherol
(pharmacology)
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