Evaluation of primary DNA damage, cytogenetic biomarkers and genetic polymorphisms for CYP1A1 and GSTM1 in road tunnel construction workers.

Abstract

In tunnel construction workers, occupational exposure to dust (alpha-quartz and other particles from blasting), gases (nitrogen dioxide, NO(2)), diesel exhausts, and oil mist has been associated with lung function decline, induction of inflammatory reactions in the lungs with release of mediators that may influence blood coagulation, and increased risk of chronic obstructive pulmonary disease. The present molecular epidemiology study was designed to evaluate whether occupational exposure to indoor pollutants during road tunnel construction might result in genotoxic effects. A study group of 39 underground workers and a reference group of 34 unexposed subjects were examined. Primary and oxidative DNA damage, sister-chromatid exchanges (SCE), and micronuclei (MN) were measured in peripheral blood cells. The possible influences of polymorphisms in gene encoding for CYP1A1 and GSTM1 xenobiotic-metabolizing enzymes were also investigated. Exposure assessment was performed with detailed interviews and questionnaires. There were no significant differences in the level of primary and oxidative DNA damage and frequency of SCE between the tunnel workers and controls, whereas the frequency of MN showed a significant increase in exposed subjects compared to controls. No effects of CYP1A1 or GSTM1 variants were observed for the analyzed biomarkers. Since MN in peripheral blood lymphocytes are recognized as a predictive biomarker of cancer risk within a population of healthy subjects, the genotoxic risk of occupational exposure to various indoor environmental pollutants during road tunnel construction cannot be excluded by this biomonitoring study.

• Authors: M Villarini, M Moretti, C Fatigoni, E Agea, L Dominici, A Mattioli, R Volpi, R Pasquini
• Journal: Journal of toxicology and environmental health. Part A (J Toxicol Environ Health A) Vol. 71 Issue 21 Pg. 1430-9 ( 2008) ISSN: 1528-7394 [Print] England
• PMID: 18800292 (Publication Type: Evaluation Study, Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't)

Chemical References

• Air Pollutants, Occupational
• Dust
• Quartz
• Cytochrome P-450 CYP1A1
• Glutathione Transferase
• glutathione S-transferase M1

Topics

• Adult
• Air Pollutants, Occupational (adverse effects, blood)
• Case-Control Studies
• Comet Assay (methods)
• Cytochrome P-450 CYP1A1 (drug effects, genetics)
• DNA Damage (drug effects)
• Dust
• Glutathione Transferase (drug effects, genetics)
• Humans
• Italy
• Male
• Micronucleus Tests (methods)
• Occupational Exposure (adverse effects)
• Polymorphism, Genetic
• Quartz (adverse effects)
• Sister Chromatid Exchange (drug effects)
• Surveys and Questionnaires
• Transportation