Immunohistochemical evidence of ubiquitous distribution of the metalloendoprotease insulin-degrading enzyme (IDE; insulysin) in human non-malignant tissues and tumor cell lines.

Abstract

Immunohistochemical evidence of ubiquitous distribution of the metalloprotease insulin-degrading enzyme (IDE; insulysin) in human non-malignant tissues and tumor cells is presented. Immunohistochemical staining was performed on a multi-organ tissue microarray (pancreas, lung, kidney, central/peripheral nervous system, liver, breast, placenta, myocardium, striated muscle, bone marrow, thymus, and spleen) and on a cell microarray of 31 tumor cell lines of different origin, as well as trophoblast cells and normal blood lymphocytes and granulocytes. IDE protein was expressed in all the tissues assessed and all the tumor cell lines except for Raji and HL-60. Trophoblast cells and granulocytes, but not normal lymphocytes, were also IDE-positive.

• Authors: Gregor Weirich, Karin Mengele, Christina Yfanti, Apostolos Gkazepis, Daniela Hellmann, Anita Welk, Cecylia Giersig, Wen-Liang Kuo, Marsha Rich Rosner, Wei-Jen Tang, Manfred Schmitt
• Journal: Biological chemistry (Biol Chem) Vol. 389 Issue 11 Pg. 1441-5 (Nov 2008) ISSN: 1431-6730 [Print] Germany
• PMID: 18783335 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Insulysin

Topics

• Cell Line, Tumor
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Insulysin (analysis, blood, immunology, metabolism)
• Organ Specificity