Striatal 19S Rpt6 deficit is related to alpha-synuclein accumulation in MPTP-treated mice.

Abstract

Striatal mitochondrial proteins were investigated using proteomics in the 1-methyl 4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson's disease. Four proteins, 19S proteasome ATPase Rpt6 (19S Rpt6), Lectin-related nature killer cell receptor LY 49S, Zinc finger A20 domain containing 1, and the sodium channel-associated protein 1 isoform 2, were significantly decreased while alpha-synuclein was increased in MPTP-treated mice. The altered levels of 19S Rpt6 and alpha-synuclein were further verified by Western blot. Experiments using small interfering RNA (siRNA) showed that alpha-synuclein was increased by 50% in cultured striatal neurons when 19S Rpt6 was knocked down. Taken together, our results imply that a deficiency in 19S Rpt6 may be partially related to the MPTP-induced increase in alpha-synuclein in the striatum.

• Authors: Bin Liu, Qiaoqiao Shi, Shiping Ma, Nan Feng, Jiang Li, Ling Wang, Xiaoliang Wang
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 376 Issue 2 Pg. 277-82 (Nov 14 2008) ISSN: 1090-2104 [Electronic] United States
• PMID: 18782562 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Ly
• Lectins, C-Type
• Neurotoxins
• Receptors, NK Cell Lectin-Like
• alpha-Synuclein
• 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
• Adenosine Triphosphatases

Topics

• 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (pharmacology)
• Adenosine Triphosphatases (genetics, metabolism)
• Animals
• Antigens, Ly (metabolism)
• Corpus Striatum (drug effects, metabolism, pathology)
• Lectins, C-Type (metabolism)
• Male
• Mice
• Mice, Inbred C57BL
• Neurons (drug effects, metabolism, pathology)
• Neurotoxins (toxicity)
• Parkinson Disease, Secondary (chemically induced, genetics, metabolism)
• Proteomics
• RNA Interference
• Receptors, NK Cell Lectin-Like
• alpha-Synuclein (metabolism)