Intrastriatal injection of hypoxanthine alters striatal ectonucleotidase activities: a time-dependent effect.

Abstract

The aim of this study was to investigate the effects of intrastriatal injection of hypoxanthine on ectonucleotidase (E-NTPDases and ecto-5'-nucleotidase) activities and expressions in the striatum of rats. The effect of pre-treatment with vitamins E and C on the effects elicited by this oxypurine on enzymatic activities and on thiobarbituric reactive substances (TBARS) was also investigated. The effect of pre-incubation with hypoxanthine on nucleotide hydrolysis in striatum homogenate was also determined. Adult Wistar rats were divided into (1) control and (2) hypoxanthine-injected groups. For ectonucleotidase activity determination, the animals were sacrificed at 30 min, 24 h and 7 days after drug infusion. For the evaluation of the expression of NTPDase 1-3 and also ecto-5'-nucleotidase, TBARS assay and the influence of the pre-treatment with vitamins on ectonucleotidase activities, the animals were sacrificed 24 h after hypoxanthine infusion. Results show that hypoxanthine infusion significantly inhibited ectonucleotidase activities and increased TBARS only 24 h after administration. Pre-treatment with vitamins was able to prevent these effects. Moreover, ecto-5'-nucleotidase expression was increased (80%) at 24 h after hypoxanthine infusion. We suggest that these hypoxanthine-induced biochemical modifications could, at least in part, participate in the pathophysiology of Lesch Nyhan disease.

• Authors: Caren S Bavaresco, Fabria Chiarani, Janaina Kolling, Denise B Ramos, Giana P Cognato, Carla D Bonan, Maurício R Bogo, João J F Sarkis, Carlos A Netto, Angela T S Wyse
• Journal: Brain research (Brain Res) Vol. 1239 Pg. 198-206 (Nov 06 2008) ISSN: 1872-6240 [Electronic] Netherlands
• PMID: 18775418 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD
• Central Nervous System Agents
• Nucleotides
• Thiobarbituric Acid Reactive Substances
• Vitamins
• Vitamin E
• Hypoxanthine
• 5'-Nucleotidase
• Adenosine Triphosphatases
• Pyrophosphatases
• ectoATPase
• nucleoside-triphosphate diphosphohydrolase 3
• Apyrase
• CD39 antigen
• Ascorbic Acid

Topics

• 5'-Nucleotidase (metabolism)
• Adenosine Triphosphatases (metabolism)
• Animals
• Antigens, CD (metabolism)
• Apyrase (metabolism)
• Ascorbic Acid (administration & dosage, pharmacology)
• Central Nervous System Agents (administration & dosage, pharmacology)
• Corpus Striatum (drug effects, enzymology, metabolism)
• Hydrolysis (drug effects)
• Hypoxanthine (administration & dosage, pharmacology)
• Male
• Nucleotides (metabolism)
• Pyrophosphatases (metabolism)
• Rats
• Rats, Wistar
• Thiobarbituric Acid Reactive Substances (metabolism)
• Time Factors
• Vitamin E (administration & dosage, pharmacology)
• Vitamins (administration & dosage, pharmacology)