Two female Chinese Nongda miniature pigs underwent gastric and colonic lavage with tap water followed by disinfection of mucosa with 1:10 iodophor. An
endoscope was inserted and the colonic wall was punctured with a needle knife, dilated with a balloon-dilator and a double-channel
endoscope was advanced into the peritoneal cavity. Under direct observation through this
endoscope, a trans-gastric entrance was made with the second double-channel
endoscope. With the help of transcolonic
endoscope, the left Fallopian tube was ligated and partially resected using the transgastric
endoscope. With the help of transgastric
endoscope, liver biopsy was performed using the transcolonic
endoscope. Finally, the gastric incision was closed with 3 clips and the colonic incision was closed with a loop and a
clip.
Antibiotics were used for 3 days following the procedures. Seventeen days later
laparotomy was performed to observe the
infection, visceral damage and adhesion, healing of the incisions of gastrointestinal duct, etc.
RESULTS: Compared with the single route, the dual routes were more convenient to perform the liver biopsy and
salpingectomy. The pigs drank and ate normally soon after the
resuscitation. The pigs looked well and gained weight during 2 weeks after the operation. Repeat endoscopy in 2 weeks showed a well-healed gastric incision with 2 clips still in place and a healed colonic incision with 1
clip still attached. The necropsy revealed a complete transmural healing of the gastric incision with minimal adhesion and a complete healing of the colonic incision without any adhesion. Few adhesions were found around the liver biopsy site and the
salpingectomy site without any intraperitoneal
infection or organ damage.
CONCLUSION: Combined transgastric and transcolonic approach appears safe and feasible and facilitates translumenal intraperitoneal interventions.