Liquid chromatography-tandem mass spectrometry method for routine measurement of oxalic acid in human plasma.

Abstract

A solid phase extraction (SPE)-LC-MSMS method for the routine determination of oxalic acid (OX) in plasma, a diagnostic marker of primary hyperoxaluria (PH), was developed and validated. The normal range of OX was found to be 3-11 micromol/L (n=67), with no differences attributable to gender or age. The effect of pre-analytical factors on the in vitro production of OX was investigated, and plasma was found to be stable for 1-2 h at room temperature, less after ingestion of vitamin C; the process was not completely stopped by preservation at either -20 or -70 degrees C.

• Authors: Katja Benedikte Presto Elgstoen
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 873 Issue 1 Pg. 31-6 (Sep 15 2008) ISSN: 1570-0232 [Print] Netherlands
• PMID: 18755640 (Publication Type: Evaluation Study, Journal Article, Validation Study)

Chemical References

• Oxalic Acid

Topics

• Chromatography, High Pressure Liquid (methods)
• Drug Stability
• Freezing
• Humans
• Hyperoxaluria, Primary (blood)
• Oxalic Acid (blood)
• Reference Values
• Solid Phase Extraction (methods)
• Specimen Handling
• Tandem Mass Spectrometry (methods)