Abstract | BACKGROUND AND PURPOSE: METHODS: Adult mice, subjected to 30 min of intraluminal middle cerebral artery occlusion, were intraperitoneally treated with GHB (100 mg/kg, twice/day, 8 h apart) or saline for 10 days. Motor recovery was evaluated by the grip strength test. The brain lesion was assessed by cresyl violet and NeuN staining 5 weeks after stroke. Expression of neuroplasticity-related genes (GAP43, c-jun, neurocan and ephrin B1) was analyzed by Taqman real-time PCR. RESULTS: GHB-treated mice regained their body weight faster and recovered grip strength (3 weeks after stroke) more quickly than saline-treated mice. This was noteworthy as GHB did not influence ischemia-induced brain injury, as revealed by cresyl violet and neuronal staining. The Taqman PCR assay revealed a decreased expression of c-jun and neurocan in the ischemic striatum of GHB-treated mice in comparison to saline-treated mice. CONCLUSION: GHB at a low dose accelerates neurological recovery following ischemic stroke. Further studies are necessary to determine the potential relationship between GHB, neuroplasticity, sleep and stroke recovery.
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Authors | Bo Gao, Ertugrul Kilic, Christian R Baumann, Dirk M Hermann, Claudio L Bassetti |
Journal | Cerebrovascular diseases (Basel, Switzerland)
(Cerebrovasc Dis)
Vol. 26
Issue 4
Pg. 413-9
( 2008)
ISSN: 1421-9786 [Electronic] Switzerland |
PMID | 18753748
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright 2008 S. Karger AG, Basel. |
Chemical References |
- Adjuvants, Anesthesia
- Efnb1 protein, mouse
- Ephrin-B1
- Ncan protein, mouse
- Nerve Tissue Proteins
- Neurocan
- Proteoglycans
- Sodium Oxybate
- JNK Mitogen-Activated Protein Kinases
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Topics |
- Adjuvants, Anesthesia
(pharmacology)
- Animals
- Brain Ischemia
(drug therapy)
- Disease Models, Animal
- Ephrin-B1
(genetics)
- Gene Expression
(drug effects)
- Infarction, Middle Cerebral Artery
(drug therapy)
- JNK Mitogen-Activated Protein Kinases
(genetics)
- Male
- Mice
- Mice, Inbred C57BL
- Nerve Tissue Proteins
(genetics)
- Neurocan
- Neuronal Plasticity
(drug effects, physiology)
- Proteoglycans
(genetics)
- Recovery of Function
(drug effects, physiology)
- Sodium Oxybate
(pharmacology)
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