Restoration of FGF receptor type 2 enhances radiosensitivity of hormone-refractory human prostate carcinoma PC-3 cells.

Abstract

BACKGROUND:

This study was undertaken to investigate the radiosensitizing effects of fibroblast growth factor receptor 2IIIb (FGFR2IIIb) in androgen-independent human prostate carcinoma PC-3 cells devoid of normally resident epithelial cell FGFR2IIIb.

MATERIALS AND METHODS:

A clonal line of PC-3 cells expressing FGFR2IIIb was established by stable transfection. Clonogenic cell survival, apoptosis and cell cycle distribution with and without gamma-irradiation were then compared between FGFR2IIIb-expressing PC-3 cells and control cells mock-transfected with vector alone.

RESULTS:

Gamma-irradiation resulted in an increase of clonogenic cell death concurrent with enhanced apoptosis and cell cycle arrest in the G2/M-phase in both transfected and untransfected cells. A quantitative analysis of all three parameters indicated that cells expressing FGFR2IIIb were significantly more sensitive to irradiation than control cells.

CONCLUSION:

These results indicate that restoration of FGFR2IIIb to PC-3 cells enhances their sensitivity to irradiation through acceleration of apoptosis and cell cycle arrest.

• Authors: Akio Matsubara, Jun Teishima, Suichinov Mirkhat, Hiroaki Yasumoto, Hideki Mochizuki, Mitsuhiro Seki, Kazuaki Mutaguchi, Wallace L Mckeehan, Tsuguru Usui
• Journal: Anticancer research (Anticancer Res) 2008 Jul-Aug Vol. 28 Issue 4B Pg. 2141-6 ISSN: 0250-7005 [Print] Greece
• PMID: 18751387 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Receptor, Fibroblast Growth Factor, Type 2
• keratinocyte growth factor receptor

Topics

• Apoptosis (physiology, radiation effects)
• Cell Division (physiology, radiation effects)
• Cell Line, Tumor
• G2 Phase (physiology, radiation effects)
• Gamma Rays
• Humans
• Male
• Neoplasms, Hormone-Dependent (genetics, metabolism, pathology, radiotherapy)
• Prostatic Neoplasms (genetics, metabolism, pathology, radiotherapy)
• Radiation Tolerance
• Receptor, Fibroblast Growth Factor, Type 2 (biosynthesis, genetics, physiology)
• Transfection