Inhibition of the growth of human hepatoma cell line bothin vitro andin vivo by transducing CKI genep21 ( WAF-1 ) with GE7 targeting gene delivery system.

Abstract

The EGF receptor-mediated targeting gene delivery system GE7 was used to transduce exogenous genepCEP-p21 ( WAF-1 ) into human hepatocellular carcinoma cell bothin vitro andin vivo. Afterin vitro transduction of the exogenous gene, the growth of the cell lines SMMC-7721 and BEL-7402 was significantly inhibited compared with the control. On day 8 the inhibition rates of the above cell lines reached 56.0% and 66.7%, respectively. Thein vivo experiment showed that the growth of human hepatoma transplanted in nude mice injected with GE7 gene delivery system subcutaneously once a week for 3 weeks was remarkably inhibited compared with that of untransfected control. The average tumor weight of the experiment group was (0.083 +/-0.043) g, while that of the control group was (0.281 +/-0.173) g. The difference is significant (P<0.05). It was indicated that GE7 gene delivery system could efficiently transduce exogenous genepCEP-p21 ( WAF-1 ) into hepatoma cell with high EGF receptor expression, and inhibit the cell growth with high efficacy bothin vivo andin vitro.

• Authors: J Han, P Tian, X Liu, M Yao, J Gu
• Journal: Science in China. Series C, Life sciences (Sci China C Life Sci) Vol. 43 Issue 6 Pg. 663-8 (Dec 2000) ISSN: 1006-9305 [Print] China
• PMID: 18726362 (Publication Type: Journal Article)