Thermosensitive polymer-conjugated albumin nanospheres as thermal targeting anti-cancer drug carrier.

Abstract

Thermosensitive Poly(N-isopropylacrylamide-co-acrylamide-co-allylamine) (PNIPAM-AAm-AA)-conjugated albumin nanospheres (PAN) was developed as a new thermal targeting anti-cancer drug carrier by conjugating PNIPAM-AAm-AA on the surface of albumin nanospheres (AN). AN with diameter below 200nm and narrow size distribution was successfully prepared in the first step with desolvation technique. PNIPAM-AAm-AA with different molecular weight (M(w)) was synthesized in the second step by radical polymerization and conjugated onto the surface of AN. Anti-cancer drug adriamycin (ADR) was then entrapped into the AN and PAN during the particle preparation. Compared with AN, the release rate of ADR from PAN in trypsin solution was slower, and decreased with increasing the conjugation amounts (hairy density) or M(w) of PNIPAM-AAm-AA (hairy length). Moreover, the release of ADR from PAN above the cloud-point temperature (T(cp)) of PNIPAM-AAm-AA became faster due to shrinkage of hairy thermosensitive polymer. To testify the thermal targetability in vivo, PAN was incubated with HepG2 cells. As expected, PAN can target cancer cells above the T(cp) of PNIPAM-AAm-AA, whereas it cannot below the T(cp). These results might reflect that PAN may selectively accumulate onto solid tumors that are maintained above physiological temperature due to local hyperthermia.

• Authors: Zheyu Shen, Wei Wei, Yongjiang Zhao, Guanghui Ma, Toshiaki Dobashi, Yasuyuki Maki, Zhiguo Su, Jinpei Wan
• Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (Eur J Pharm Sci) Vol. 35 Issue 4 Pg. 271-82 (Nov 15 2008) ISSN: 0928-0987 [Print] Netherlands
• PMID: 18721874 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acrylic Resins
• Albumins
• Antineoplastic Agents
• Drug Carriers
• Polymers
• poly(N-isopropylacrylamide-co-acrylamide-co-allylamine)
• poly-N-isopropylacrylamide
• Doxorubicin

Topics

• Acrylic Resins (chemistry)
• Albumins (chemistry)
• Antineoplastic Agents (administration & dosage, chemistry)
• Cell Line
• Cell Nucleus (chemistry, metabolism)
• Doxorubicin (administration & dosage, chemistry)
• Drug Carriers (chemistry)
• Drug Compounding
• Electric Conductivity
• Fluorescence
• Humans
• Light
• Microscopy, Electron, Transmission
• Nanospheres
• Particle Size
• Polymers
• Scattering, Radiation
• Solubility
• Temperature
• Thermodynamics