[Effect of Xinnao Shutong capsule on apoptosis after cerebral ischemia and reperfusion in rats].

Abstract

OBJECTIVE:

To study apoptosis-regulating cytokines and apoptosis on focal cerebral ischemia and reperfusion in rats treated with Xinnao Shutong capsule.

METHOD:

Rat models of focal cerebral ischemia and reperfusion were established by thread ligation in middle cerebral artery occlusions (MCAO). After 24 hours, the brains were removed to detect changes of protein expression of Bax, Bcl-2, Fas, Fas-L and caspase-3 by immuno-hisochemistry, and apoptosis of cortical neurons by TUNEL RESULT: Compared to control, brain cortex have decreasing the protein expression of Bcl-2 and the ratio of Bcl-2/Bax, increasing the protein expression of Bax, Fas, Fas-L and caspase-3 of ischemia and reperfusion models group (P < 0.01). Xinnao Shutong capsule group could increase the protein expression of Bcl-2 and the ratio of Bcl-2/Bax, and obviously decrease the protein expression of Bax, Fas, Fas-L and caspase-3, then reduce the number of apoptotic cells of cortex (P < 0.01).

CONCLUSION:

Xinnao Shutong capsule protect injured rat brain tissue, may be related to decrease neuronal apoptosis and adjusted protein expression of apoptosis-regulating cytokines.

• Authors: Yun-Ling Zhang, Xue-Mei Liu, Hong-Sheng Liu, Jin-Li Lou, Hong Zheng, Qi-Fu Huang
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 33 Issue 10 Pg. 1188-91 (May 2008) ISSN: 1001-5302 [Print] China
• PMID: 18720874 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apoptosis Regulatory Proteins
• Capsules
• Drugs, Chinese Herbal
• Xinnao Shutong

Topics

• Animals
• Apoptosis (drug effects)
• Apoptosis Regulatory Proteins (genetics, metabolism)
• Brain Ischemia (drug therapy, metabolism, surgery)
• Capsules (administration & dosage)
• Cerebral Infarction (drug therapy, metabolism, surgery)
• Disease Models, Animal
• Drugs, Chinese Herbal (administration & dosage)
• Female
• Gene Expression (drug effects)
• Humans
• Male
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion