Wuweizisu C from Schisandra chinensis decreases membrane potential in C6 glioma cells.

Abstract

AIM:

To study the effects of dibenzocyclooctadiene lignans isolated from Schisandra chinensis, such as wuweizisu C, gomisin N, gomisin A, and schisandrin, on the membrane potential in C6 glioma cells.

METHODS:

The membrane potential was estimated by measuring the fluorescence change in DiBAC-loaded glioma cells.

RESULTS:

Wuweizisu C decreased the membrane potential in a concentration-dependent manner. Gomisin N and gomisin A, however, showed differential modulation and no change was induced by schisandrin or dimethyl- 4,4'-dimethoxy-5,6,5',6'-dimethylene dioxybiphenyl-2,2'-dicarboxylate, a synthetic drug derived from dibenzocyclooctadiene lignans. We found no involvement of G(i/o ) proteins, phospholipase C, and extracellular Na(+) on the wuweizisu C-induced decrease of the membrane potential. Wuweizisu C by itself did not change the intracellular Ca(2+)[Ca(2+)](i) concentration, but decreased the ATP-induced Ca(2+) increase in C6 glioma cells. The 4 lignans at all concentrations used in this study did not induce any effect on cell viability. Furthermore, we found a similar decrease of the membrane potential by wuweizisu C in PC12 neuronal cells.

CONCLUSION:

Our results suggest that the decrease in the membrane potential and the modulation of [Ca(2+)](i) concentration by wuweizisu C could be important action mechanisms of wuweizisu C.

• Authors: Young-whan Choi, Kyeok Kim, Ji-yeong Jo, Hyo-lim Kim, You-jin Lee, Woo-jung Shin, Santosh-J Sacket, Mijin Han, Dong-soon Im
• Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin) Vol. 29 Issue 9 Pg. 1006-12 (Sep 2008) ISSN: 1745-7254 [Electronic] United States
• PMID: 18718168 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cyclooctanes
• Indicators and Reagents
• Lignans
• Polycyclic Compounds
• schizandrin C
• Type C Phospholipases
• GTP-Binding Proteins
• Calcium

Topics

• Animals
• Calcium (metabolism)
• Cell Line, Tumor
• Cyclooctanes (pharmacology)
• Fruit (chemistry)
• GTP-Binding Proteins (metabolism)
• Glioma (physiopathology)
• Humans
• Indicators and Reagents
• Lignans (pharmacology)
• Membrane Potentials (drug effects)
• PC12 Cells
• Polycyclic Compounds (pharmacology)
• Rats
• Schisandra (chemistry)
• Type C Phospholipases (metabolism)