Selection of antigens and development of prototype tests for point-of-care leprosy diagnosis.

Abstract

Leprosy can be a devastating chronic infection that causes nerve function impairment and associated disfigurement. Despite the recent reduction in the number of registered worldwide leprosy cases as a result of the widespread use of multidrug therapy, the number of new cases detected each year remains relatively stable. The diagnosis of leprosy is currently based on the appearance of clinical signs and requires expert clinical, as well as labor-intensive and time-consuming laboratory or histological, evaluation. For the purpose of developing an effective, simple, rapid, and low-cost diagnostic alternative, we have analyzed the serologic antibody response to identify Mycobacterium leprae proteins that are recognized by leprosy patients. More than 100 recombinant antigens were analyzed in a protein array format to select those with discriminatory properties for leprosy diagnosis. As expected, multibacillary leprosy patients recognized more antigens with stronger antibody responses than paucibacillary leprosy patients. Our data indicate, however, that multibacillary patients can be distinguished from paucibacillary patients, and both of these groups can be segregated from endemic control groups. We went on to confirm the diagnostic properties of antigens ML0405 and ML2331 and the LID-1 fusion construct of these two proteins by enzyme-linked immunosorbent assay. We then demonstrated the performance of these antigens in rapid test formats with a goal of developing a point-of-care diagnostic test. A serological diagnostic test capable of identifying and allowing treatment of leprosy could reduce transmission, prevent functional disabilities and stigmatizing deformities, and facilitate leprosy eradication.

• Authors: Malcolm S Duthie, Greg C Ireton, Ganga V Kanaujia, Wakako Goto, Hong Liang, Ajay Bhatia, Jean Marie Busceti, Murdo Macdonald, Kapil Dev Neupane, Chaman Ranjit, Bishwa Raj Sapkota, Marivic Balagon, Javan Esfandiari, Darrick Carter, Steven G Reed
• Journal: Clinical and vaccine immunology : CVI (Clin Vaccine Immunol) Vol. 15 Issue 10 Pg. 1590-7 (Oct 2008) ISSN: 1556-679X [Electronic] United States
• PMID: 18716007 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Validation Study)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Recombinant Proteins

Topics

• Adolescent
• Adult
• Aged
• Antibodies, Bacterial (blood)
• Antigens, Bacterial
• Female
• Humans
• Leprosy (diagnosis)
• Male
• Middle Aged
• Mycobacterium leprae (isolation & purification)
• Point-of-Care Systems
• Recombinant Proteins