Abstract | BACKGROUND AND AIM: METHODS: Two hundred and sixteen cases of newly diagnosed T2DM patients (104 cases complicated with NAFLD) and 108 cases of normal glucose tolerances (NGT) were recruited. Hemi-nested polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and PCR-direct sequence analysis were conducted to detect the polymorphism of LEPR G3057A variation. Plasma leptin levels were measured by enzyme-linked immunosorbent assay kit. Plasma lipid and glucose metabolic parameters were measured routinely. Liver ultrasound was carried out for all subjects. RESULTS: T2DM patients complicated with NAFLD had higher plasma insulin, leptin, triglycerides (TG) and low-density lipoprotein cholesterol ( LDL-C) levels than those without NAFLD and NGT subjects. The variant frequency at nucleotide 3057 G-->A transversion was 76.0% in type 2 diabetic patients complicated with NAFLD, which was also significantly higher than those without NAFLD (62.1%) and NGT cases (53.2%). There was also significant difference in genotype distribution between the three groups (chi(2) = 14.63, P < 0.01). CONCLUSION: The polymorphism of LEPR gene 3057 probably contributes to the onset of NAFLD by regulating lipid metabolism and affecting insulin sensitivity.
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Authors | Hongyun Lu, Jiazhong Sun, Liao Sun, Xiaochun Shu, Yancheng Xu, Danhong Xie |
Journal | Journal of gastroenterology and hepatology
(J Gastroenterol Hepatol)
Vol. 24
Issue 2
Pg. 228-32
(Feb 2009)
ISSN: 1440-1746 [Electronic] Australia |
PMID | 18713300
(Publication Type: Journal Article)
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Chemical References |
- Blood Glucose
- Cholesterol, LDL
- Insulin
- LEPR protein, human
- Leptin
- Receptors, Leptin
- Triglycerides
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Topics |
- Aged
- Asian People
(genetics)
- Blood Glucose
(analysis)
- Case-Control Studies
- China
(epidemiology)
- Cholesterol, LDL
(blood)
- DNA Mutational Analysis
- Diabetes Complications
(blood, ethnology, genetics)
- Diabetes Mellitus, Type 2
(blood, complications, ethnology, genetics)
- Fatty Liver
(blood, ethnology, genetics)
- Female
- Gene Frequency
- Genetic Predisposition to Disease
- Humans
- Insulin
(blood)
- Leptin
(blood)
- Liver
(chemistry, diagnostic imaging)
- Logistic Models
- Male
- Middle Aged
- Phenotype
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Receptors, Leptin
(genetics)
- Risk Assessment
- Risk Factors
- Triglycerides
(blood)
- Ultrasonography
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