| Abstract | Owing to its diverse sex types, the cucumber plant has been studied widely as a model for sex determination. In addition to environmental factors and plant hormones, three major genes-F/f, M/m, and A/a-regulate the sex types in the cucumber plant. By combining the bulked segregant analysis (BSA) and the sequence-related amplified polymorphism (SRAP) technology, we identified eight markers linking to the M/m locus. Among them, the two closely linked SRAP markers flanking the M/m locus were the co-dominant marker ME1EM26 and the dominant marker ME1EM23. Further, the co-dominant marker ME8SA7 co-segregated with the M/m locus. With the chromosome walking method using the cucumber genomic bacterial artificial chromosome (BAC) library, we successfully developed a co-dominant SCAR marker S_ME1EM23 from the ME1EM23 sequence. Along with the other two co-dominant SCAR markers S_ME1EM26 and S_ME8SA7 (developed from ME1EM26 and ME8SA7, respectively) in a larger segregating population (900 individuals), the M/m locus was mapped between S_ME1EM26 (5.4 cM) and S_ME1EM23 (0.7 cM), and S_ME8SA7 co-segregated with it. |
| Authors | Zheng Li, Junsong Pan, Yuan Guan, Qianyi Tao, Huanle He, Longting Si, Run Cai
(Affiliation: Shanghai Jiaotong University, School of Agriculture and Biology, Shanghai, China.)
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| Journal | TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
(Theor Appl Genet)
Vol. 117
Issue 8
Pg. 1253-60
(Nov 2008)
ISSN: 0040-5752 Germany |
| PMID | 18712515
(Publication Type: Journal Article)
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