Targeting myelin proteolipid protein to the MHC class I pathway by ubiquitination modulates the course of experimental autoimmune encephalomyelitis.

Abstract

Relapsing-remitting experimental autoimmune encephalomyelitis (EAE), a multiple sclerosis model, is induced in mice by injection of myelin proteolipid protein (PLP) encephalitogenic peptide, PLP139-151, in adjuvant. In this study, prior to EAE induction, mice were vaccinated with a bacterial plasmid encoding a PLP-ubiquitin fusion (pCMVUPLP). During the relapse phase of EAE, clinical signs, histopathologic changes, in vitro lymphoproliferation to PLP139-151 and interferon-gamma levels were reduced in pCMVUPLP-vaccinated mice, compared to mock-vaccinated mice (controls). Lymphocytes from pCMVUPLP-vaccinated mice produced interleukin-4, a cytokine lacking in controls. Thus, pCMVUPLP vaccination can modulate the relapse after EAE induction.

• Authors: Diethilde J Theil, Jane E Libbey, Fernando Rodriguez, J Lindsay Whitton, Ikuo Tsunoda, Tobias J Derfuss, Robert S Fujinami
• Journal: Journal of neuroimmunology (J Neuroimmunol) Vol. 204 Issue 1-2 Pg. 92-100 (Nov 15 2008) ISSN: 0165-5728 [Print] Netherlands
• PMID: 18706703 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Cytokines
• Histocompatibility Antigens Class I
• Myelin Proteolipid Protein
• Peptide Fragments
• myelin proteolipid protein (139-151)

Topics

• Analysis of Variance
• Animals
• Body Weight
• Cell Proliferation (drug effects)
• Cytokines (metabolism)
• Disease Models, Animal
• Encephalomyelitis, Autoimmune, Experimental (chemically induced, immunology, physiopathology, prevention & control)
• Histocompatibility Antigens Class I (genetics, metabolism)
• Lymphocyte Activation (drug effects, immunology)
• Mice
• Molecular Sequence Data
• Myelin Proteolipid Protein (genetics, immunology)
• Peptide Fragments
• Severity of Illness Index
• Signal Transduction (drug effects, physiology)
• Spinal Cord (metabolism, pathology)
• T-Lymphocytes (drug effects, immunology, metabolism)
• Time Factors
• Ubiquitination (drug effects, physiology)