Abstract |
Hyoscyamus niger L. is a medicinal plant which produces a class of jasmonate-responsive pharmaceutical secondary metabolites named as tropane alkaloids. As a family of signaling phytohormones, jasmonates play significant roles in the biosynthesis of many plant secondary metabolites. In jasmonate biosynthetic pathway of plants, allene oxide cyclase (AOC, [...] EC 5.3.99.6 [...]) catalyzes the most important step. Here we cloned a cDNA from H. niger, named HnAOC (GenBank accession: AY708383), which was 1044 bp long, with a 747 bp open reading frame (ORF) encoding a polypeptide of 248 amino acid residues. Southern blot analysis indicated that it was a multi-copy gene. RT-PCR analysis revealed that the expression of HnAOC was regulated by various stresses and elicitors, with methyl-jasmonate showing the most prominent inducement. The characterization of HnAOC would be helpful for improving the production of valuable secondary metabolites by regulating the biosynthesis ofjasmonates.
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Authors | K Jiang, Z Liao, Y Pi, Z Huang, R Hou, Y Cao, Q Wang, Z Sun, K Tang |
Journal | Molekuliarnaia biologiia
(Mol Biol (Mosk))
Vol. 42
Issue 3
Pg. 434-44
( 2008)
ISSN: 0026-8984 [Print] Russia (Federation) |
PMID | 18702301
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Alkaloids
- Cyclopentanes
- DNA, Complementary
- Oxylipins
- Plant Proteins
- jasmonic acid
- Intramolecular Oxidoreductases
- hydroperoxide isomerase
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Topics |
- Alkaloids
(genetics, metabolism)
- Base Sequence
- Cloning, Molecular
- Cyclopentanes
(metabolism)
- DNA, Complementary
(genetics)
- Gene Expression Regulation, Enzymologic
(physiology)
- Gene Expression Regulation, Plant
(physiology)
- Hyoscyamus
(enzymology, genetics)
- Intramolecular Oxidoreductases
(biosynthesis, genetics)
- Molecular Sequence Data
- Oxylipins
(metabolism)
- Plant Proteins
(biosynthesis, genetics)
- Plants, Medicinal
(genetics, metabolism)
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