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[Molecular cloning and expression profile of a jasmonate biosynthetic pathway gene allene oxide cyclase from Hyoscyamus niger].

Abstract
Hyoscyamus niger L. is a medicinal plant which produces a class of jasmonate-responsive pharmaceutical secondary metabolites named as tropane alkaloids. As a family of signaling phytohormones, jasmonates play significant roles in the biosynthesis of many plant secondary metabolites. In jasmonate biosynthetic pathway of plants, allene oxide cyclase (AOC, [...] EC 5.3.99.6 [...]) catalyzes the most important step. Here we cloned a cDNA from H. niger, named HnAOC (GenBank accession: AY708383), which was 1044 bp long, with a 747 bp open reading frame (ORF) encoding a polypeptide of 248 amino acid residues. Southern blot analysis indicated that it was a multi-copy gene. RT-PCR analysis revealed that the expression of HnAOC was regulated by various stresses and elicitors, with methyl-jasmonate showing the most prominent inducement. The characterization of HnAOC would be helpful for improving the production of valuable secondary metabolites by regulating the biosynthesis ofjasmonates.
AuthorsK Jiang, Z Liao, Y Pi, Z Huang, R Hou, Y Cao, Q Wang, Z Sun, K Tang
JournalMolekuliarnaia biologiia (Mol Biol (Mosk)) Vol. 42 Issue 3 Pg. 434-44 ( 2008) ISSN: 0026-8984 [Print] Russia (Federation)
PMID18702301 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Alkaloids
  • Cyclopentanes
  • DNA, Complementary
  • Oxylipins
  • Plant Proteins
  • jasmonic acid
  • Intramolecular Oxidoreductases
  • hydroperoxide isomerase
Topics
  • Alkaloids (genetics, metabolism)
  • Base Sequence
  • Cloning, Molecular
  • Cyclopentanes (metabolism)
  • DNA, Complementary (genetics)
  • Gene Expression Regulation, Enzymologic (physiology)
  • Gene Expression Regulation, Plant (physiology)
  • Hyoscyamus (enzymology, genetics)
  • Intramolecular Oxidoreductases (biosynthesis, genetics)
  • Molecular Sequence Data
  • Oxylipins (metabolism)
  • Plant Proteins (biosynthesis, genetics)
  • Plants, Medicinal (genetics, metabolism)

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