Effect of Tilia x viridis flower extract on the proliferation of a lymphoma cell line and on normal murine lymphocytes: contribution of monoterpenes, especially limonene.
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| Abstract |
Tilia species are widely used in Europe as medicinal plants. The selective antiproliferative activity of a Tilia cordata flower dichloromethane extract (DME) on a lymphoma cell line has been reported previously and in order to extend this to other unstudied Tilia species, the effect of Tilia x viridis DME on the proliferation of tumor and normal murine lymphocytes was investigated. The bioguided fractionation of DME yielded a fraction rich in limonene (L), alpha-pinene and beta-pinene, which presented a selective antiproliferative action on tumor lymphocytes (EC(50) on tumor cells: 3.8 +/- 0.2 microg/mL; EC(50) on normal cells: 205 +/- 1.8 microg/mL). While all monoterpenes exhibited this activity, limonene proved to be the most active (EC(50) on tumor cells: 35 +/- 2.0 microg/mL; EC(50) on normal cells: 72 +/- 5.0 microg/mL) also exerting a stimulatory effect on non-mitogen stimulated lymphocytes proliferation (% of stimulation respect to control) (mean +/- SEM): L 10 microg/mL: 25 +/- 1.0%; 20 microg/mL: 38.5 +/- 2.5%; L 40 microg/mL: 41 +/- 0.9%; L 60 microg/mL: 58.5 +/- 3%. T. x viridis may thus constitute a potential source of monoterpenes with immunomodulatory activity.
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| Authors | Maria Gabriela Manuele, Graciela Ferraro, Claudia Anesini |
| Journal | Phytotherapy research : PTR (Phytother Res) Vol. 22 Issue 11 Pg. 1520-6 (Nov 2008) ISSN: 1099-1573 [Electronic] England |
| PMID | 18688792 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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