Protective effects of caffeic acid phenethyl ester on intestinal ischemia-reperfusion injury.

Abstract

AIM:

Intestinal ischemia reperfusion (IR) causes tissue injury in two ways, starting a pro-inflammatory cascade and oxidative stress. The aim of this study was to investigate the possible protective effects of caffeic acid phenethyl ester (CAPE), which has antioxidant and anti-inflammatory properties, against intestinal IR injury in rats.

MATERIALS AND METHODS:

Forty male Wistar-Albino rats were divided into five groups: Sham, IR, IR plus ethanol (vehicle), IR plus 10 mg/kg (IR + 10CAPE), and 30 mg/kg CAPE (IR + 30CAPE) at the 30-min ischemic period. Intestines were exteriorized and the superior mesenteric artery was occluded for 45-min ischemia and then the clamp was removed for 120-min reperfusion. After the experiment, the intestines were removed for biochemical and light microscopic examinations. Additionally, blood samples were taken for plasma TNF-alpha measurement.

RESULTS:

The TBARS levels of the IR and IR + Ethanol groups were higher than the Sham group (P < 0.05). Both CAPE treatments decreased TBARS levels in comparison with the IR group (P < 0.05). In both CAPE-treated groups, while the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were increased compared to all other groups, which was similarly the case for the CAT activity compared to the Sham and IR + Ethanol groups (P < 0.05). There were no significant differences between GSH levels of all study groups. The TNF-alpha levels of the IR and IR + Ethanol groups were non-significantly increased compared to the Sham group (P > 0.05). The TNF-alpha levels of 10 and 30 mg/kg CAPE groups were non-significantly decreased compared to the IR group (P > 0.05). The tissue MPO activities of the IR and IR + Ethanol groups were higher than the Sham group (P < 0.05). The MPO activities of the IR + 10CAPE and IR + 30CAPE groups were not significantly different from the Sham group (P > 0.05). There was necrosis of mucosa in the IR and IR + Ethanol groups in light microscopic evaluations. Those changes were significantly reversed by 30 mg/kg CAPE treatment.

CONCLUSION:

The intestinal IR injury may be reversed by anti-inflammatory and antioxidant actions of the CAPE. However, 30 mg/kg CAPE treatment may be more efficient in preventing intestinal IR injury in rats.

• Authors: Yuksel Yildiz, Mukadder Serter, Rauf Onur Ek, Kemal Ergin, Serpil Cecen, Ece Mine Demir, Cigdem Yenisey
• Journal: Digestive diseases and sciences (Dig Dis Sci) Vol. 54 Issue 4 Pg. 738-44 (Apr 2009) ISSN: 1573-2568 [Electronic] United States
• PMID: 18683050 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Caffeic Acids
• Tumor Necrosis Factor-alpha
• Malondialdehyde
• Catalase
• Peroxidase
• Glutathione Peroxidase
• Superoxide Dismutase
• caffeic acid phenethyl ester
• Glutathione
• Phenylethyl Alcohol

Topics

• Animals
• Antioxidants (metabolism)
• Caffeic Acids (therapeutic use)
• Catalase (metabolism)
• Glutathione (metabolism)
• Glutathione Peroxidase (metabolism)
• Intestinal Diseases (drug therapy, enzymology, pathology)
• Intestinal Mucosa (pathology)
• Jejunum (pathology)
• Lipid Peroxidation
• Male
• Malondialdehyde (metabolism)
• Peroxidase (metabolism)
• Phenylethyl Alcohol (analogs & derivatives)
• Rats
• Rats, Wistar
• Reperfusion Injury (drug therapy, enzymology, pathology)
• Superoxide Dismutase (metabolism)
• Tumor Necrosis Factor-alpha (blood)