Mouse protein kinase C-delta, the major isoform expressed in mouse hemopoietic cells: sequence of the cDNA, expression patterns, and characterization of the protein.

Abstract	A complementary DNA (cDNA) of 2559 bp which encode all 674 amino acids of mouse protein kinase C-delta (PKC-delta) has been isolated from a cDNA library prepared from ABPL-2, a mouse myeloid tumor. The library was screened with a partial PKC-delta cDNA clone that had been created by polymerase chain reaction (PCR) amplification of ABPL-2 RNA using primers that are conserved among all rat PKC isozymes. This approach proved to be a distinct improvement over screening with synthetic oligonucleotides. Similar sets of cDNAs prepared from other hemopoietic cell lines were screened with this PKC-delta cDNA and with probes for the other PKC isoforms. These experiments revealed that the major isoform of PKC expressed in hemopoietic cells is PKC-delta. PKC-delta protein was purified from ABPL-3, a mouse myeloid tumor which expressed principally the delta isoform of PKC. The protein eluted from a hydroxylapatite column in the same position as PKC-beta and -epsilon would elute, if present. The kinase activity of purified PKC-delta showed strict dependence on the presence of phospholipids, but showed no activation by Ca2+.
Authors	H Mischak, A Bodenteich, W Kolch, J Goodnight, F Hofer, J F Mushinski
Journal	Biochemistry (Biochemistry) Vol. 30 Issue 32 Pg. 7925-31 (Aug 13 1991) ISSN: 0006-2960 [Print] United States
PMID	1868068 (Publication Type: Comparative Study, Journal Article)
Chemical References	DNA, Neoplasm Isoenzymes Recombinant Proteins Protein Kinase C
Topics	Amino Acid Sequence Animals Base Sequence Blotting, Northern Cell Line Chromatography, Ion Exchange DNA, Neoplasm (genetics, isolation & purification) Gene Expression Gene Library Isoenzymes (genetics, isolation & purification, metabolism) Leukemia, Experimental Leukemia, Myeloid Mice Molecular Sequence Data Molecular Weight Polymerase Chain Reaction Protein Kinase C (genetics, isolation & purification, metabolism) Recombinant Proteins (isolation & purification, metabolism) Restriction Mapping Sequence Homology, Nucleic Acid

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