The RNase-T1-resistant
oligonucleotides of two Prague
Rous sarcoma viruses with temperature-sensitive (ts)
DNA polymerases (
DNA nucleotidyltransferases), termed ts LA 337 and 335 of one leukosis virus, RAV-6, and 20 of their recombinant progeny have been mapped relative to the 3'
poly (A) terminus of the
viral RNA. The resulting
oligonucleotide maps have been ocrrelated with markers of the four known viral genetic elements encoded in the
RNA of 10,000
nucleotides. In accord with previous results recombinant RNAs contained (i)
oligonucleotides characteristic of the src gene, coding for
sarcoma formation, between the
poly(A) end and 2000
nucleotides and (ii) olignucleotides characteristic of the env gene, coding for the envelope
glycoprotein, between 2500 and 5000 nucleo tides from the
poly(A) end. (iii) A cluster of four
oligonucleotides that mapped between 6000 and 8000
nucleotides from the 3'
poly(A) end of each
RNA was shared by both parental viruses and all recombinants. Since all other map segments of our recombinants failed to segregate with the ts- or wild-type markers of the parental
DNA polymerase gene (pol), it was concluded that the ts pol lesion maps in this
RNA segment. (iv) The 5' segment of each
recombinant RNA contained a cluster of four to five
oligonucleotides whose parental origin correlated with an electrophoretic marker of one of the parental virion
proteins, p27, a major product of the viral gag gene. The gene order 5'-gag-pol-env-src-poly(A) is consistent with our data.