Prevention of pathological change and cognitive degeneration of Tg2576 mice by inoculating Abeta(1-15) vaccine.

Abstract

This study aims to discuss the effect of preventing pathological changes and cognitive degeneration of Tg2576 mice by inoculating the subunit fragment of Abeta vaccine. Thirty-two Tg2576 mice were randomly divided into four groups, each having eight mice: Group I, the control group, inoculated with adjuvants; Group II, the Abeta(42) group, inoculated with Abeta(42) vaccine; Group III, the Abeta(1-15) group, inoculated with Abeta(1-15) vaccine; and Group IV, the Abeta(36-42) group, inoculated with Abeta(36-42) vaccine. The titer of the serum antibody against Abeta(42) (Group II) was significantly higher than that of the control group (Group I), and a low level of antibodies could be detected in the brain homogenate in the three vaccine-inoculated groups. Morris water maze test showed that the Abeta(42) group, Abeta(1-15) group and Abeta(36-42) group were obviously improved compared with the control group. The cultured splenocytes sampled from each group were induced by Con A or their respective antigens, and the cell proliferation of the three vaccine-inoculated groups was significantly higher than that of the control group. In the Abeta(42) group, IL2 and IFN-gamma were relatively low and IL4 and IL10 were relatively high. By contrast, IL4 and IL10 were much higher in the Abeta(1-15) group and IL2 and IFN-gamma were much higher in the Abeta(36-42) group. The immunohistochemical test showed a large number of senile plaques in the brain cortex and hippocampus of the mice in the control group, no senile plaque in the brain of the Abeta(1-15) group and Abeta(42) group mice, and a small number of senile plaques in the brain of the Abeta(36-42) group mice. The results suggest that the subunit fragment of Abeta(1-15) vaccine could prevent not only cognitive and behavioral degeneration but also Abeta deposition and formation of senile plaques in Tg2576 mice.

• Authors: Jinjia Hu, Guoying Li, Huaqiao Wang, Xian Lin, Zhibin Yao
• Journal: Science in China. Series C, Life sciences (Sci China C Life Sci) Vol. 51 Issue 8 Pg. 743-50 (Aug 2008) ISSN: 1006-9305 [Print] China
• PMID: 18677602 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amyloid beta-Peptides
• Peptide Fragments
• Vaccines
• amyloid beta-protein (1-15)

Topics

• Alzheimer Disease (pathology, physiopathology)
• Amyloid beta-Peptides (immunology, pharmacology)
• Amyloidosis (immunology, pathology, prevention & control)
• Animals
• Brain (pathology)
• Disease Models, Animal
• Humans
• Kidney (cytology)
• Liver (cytology)
• Lung (cytology)
• Maze Learning (drug effects)
• Mice
• Mice, Transgenic
• Peptide Fragments (immunology, pharmacology)
• Random Allocation
• Vaccines (immunology)