Combination chemotherapy and ALVAC-CEA/B7.1 vaccine in patients with metastatic colorectal cancer.

Abstract

PURPOSE:

The combination of vaccines and chemotherapy holds promise for cancer therapy, but the effect of cytotoxic chemotherapy on vaccine-induced antitumor immunity is unknown. This study was conducted to assess the effects of systemic chemotherapy on ALVAC-CEA/B7.1-induced T-cell immunity in patients with metastatic colorectal cancer.

EXPERIMENTAL DESIGN:

Patients with metastatic colorectal cancer were treated with fluorouracil, leucovorin, and irinotecan and were also given ALVAC-CEA/B7.1 vaccine with or without tetanus toxoid adjuvant. Eligible patients were randomized to ALVAC followed by chemotherapy and booster vaccination (group 1), ALVAC and tetanus toxoid followed by chemotherapy (group 2), or chemotherapy alone followed by ALVAC in patients without disease progression (group 3). Humoral immune responses were measured by standard ELISA assay, and carcinoembryonic antigen (CEA)-specific T-cell responses were measured by IFN-gamma enzyme-linked immunospot assay.

RESULTS:

One hundred eighteen patients were randomized to receive either ALVAC before and concomitantly with chemotherapy (n = 39), ALVAC with tetanus adjuvant before and concomitantly with chemotherapy (n = 40), or chemotherapy followed by ALVAC (n = 39). Serious adverse events were largely gastrointestinal (n = 30) and hematologic (n = 24). Overall, 42 patients (40.4%) showed objective clinical responses. All patients developed antibody responses against ALVAC, but increased anti-CEA antibody titers were detected in only three patients. Increases in CEA-specific T cells were detected in 50%, 37%, and 30% of patients in groups 1, 2, and 3, respectively. There were no differences in clinical or immune responses between the treatment groups.

CONCLUSION:

The combination of ALVAC-CEA/B7.1 vaccine and systemic chemotherapy has an acceptable safety profile in patients with metastatic colorectal cancer. Systemic chemotherapy did not affect the generation of CEA-specific T-cell responses following vaccination.

• Authors: Howard L Kaufman, Heinz-Josef Lenz, John Marshall, Deepti Singh, Chris Garett, Christine Cripps, Malcolm Moore, Margaret von Mehren, Richard Dalfen, William J Heim, Robert M Conry, Walter J Urba, Al B Benson 3rd, Maria Yu, Judy Caterini, Seunghee Kim-Schulze, Mark Debenedette, Danielle Salha, Thorsten Vogel, Ileana Elias, Neil L Berinstein
• Journal: Clinical cancer research : an official journal of the American Association for Cancer Research (Clin Cancer Res) Vol. 14 Issue 15 Pg. 4843-9 (Aug 01 2008) ISSN: 1078-0432 [Print] United States
• PMID: 18676757 (Publication Type: Clinical Trial, Phase II, Journal Article, Multicenter Study, Randomized Controlled Trial)

Chemical References

• ALVAC vaccine
• B7-1 Antigen
• Carcinoembryonic Antigen
• Viral Vaccines
• Irinotecan
• Leucovorin
• Fluorouracil
• Camptothecin

Topics

• Aged
• Antineoplastic Combined Chemotherapy Protocols (therapeutic use)
• B7-1 Antigen (chemistry)
• Camptothecin (administration & dosage, analogs & derivatives)
• Carcinoembryonic Antigen (chemistry)
• Colorectal Neoplasms (therapy)
• Female
• Fluorouracil (administration & dosage)
• Humans
• Irinotecan
• Leucovorin (administration & dosage)
• Male
• Middle Aged
• Neoplasm Metastasis
• T-Lymphocytes (metabolism)
• Treatment Outcome
• Viral Vaccines (therapeutic use)