Cellular responses to MPT-51, GlcB and ESAT-6 among MDR-TB and active tuberculosis patients in Brazil.

Abstract

Multi-drug resistant pulmonary tuberculosis (MDR-TB) may result from either insufficiency of the host cellular immune response or mycobacterial mechanisms of resistance. Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocytes from MDR-TB patients are poorly studied. The aim of this study was to evaluate CD4+IFN-gamma+, CD4+IL-10+, CD8(+)IFN-gamma+ and CD8+IL-10+ cell populations by flow cytometry in non-resistant TB and multi-drug resistant tuberculosis (MDR-TB) patients from mid-central Brazil after stimulation with MPT-51, GlcB and ESAT-6 recombinant antigens from M. tuberculosis in comparison to tuberculin skin test negative (TST) healthy individuals. Non-resistant TB patients present specific cellular responses (CD4 and CD8, both IFN-gamma and IL-10) to GlcB, MPT-51 and ESAT-6; while MDR-TB patients present only CD8+IFN-gamma+ responses to ESAT-6 and CD8+IL-10+ responses to GlcB and ESAT-6. The results show that MDR-TB patients present impaired specific CD4 IFN-gamma and IL-10 responses and increased/normal specific CD8 IFN-gamma and IL-10 responses. This suggests an important role for CD8 function in these patients.

• Authors: João Alves de Araújo-Filho, Arioldo Carvalho Vasconcelos Jr, Eduardo Martins de Sousa, André Kipnis, Elisângela Ribeiro, Ana Paula Junqueira-Kipnis
• Journal: Tuberculosis (Edinburgh, Scotland) (Tuberculosis (Edinb)) Vol. 88 Issue 5 Pg. 474-81 (Sep 2008) ISSN: 1873-281X [Electronic] Scotland
• PMID: 18676203 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Bacterial
• Bacterial Proteins
• ESAT-6 protein, Mycobacterium tuberculosis
• MPT51 protein, Mycobacterium
• Interleukin-10

Topics

• Adult
• Aged
• Antigens, Bacterial (immunology, isolation & purification)
• Bacterial Proteins (isolation & purification)
• Brazil
• CD4-CD8 Ratio
• CD4-Positive T-Lymphocytes (immunology)
• CD8-Positive T-Lymphocytes (immunology)
• Female
• Humans
• Interleukin-10 (immunology)
• Male
• Middle Aged
• Mycobacterium tuberculosis (immunology)
• Tuberculosis, Multidrug-Resistant (immunology)
• Tuberculosis, Pulmonary (immunology)