A Role for galectin-3 in renal tissue damage triggered by ischemia and reperfusion injury.

Abstract

Ischemic-reperfusion injury (IRI) triggers an inflammatory response involving neutrophils/macrophages, lymphocytes and endothelial cells. Galectin-3 is a multi-functional lectin with a broad range of action such as promotion of neutrophil adhesion, induction of oxidative stress, mastocyte migration and degranulation, and production of pro-inflammatory cytokines. The aim of this study was evaluate the role of galectin-3 in the inflammation triggered by IRI. Galectin-3 knockout (KO) and wild type (wt) mice were subjected to 45 min of renal pedicle occlusion. Blood and kidney samples were collected at 6, 24, 48 and 120 h. Blood urea was analyzed enzymatically, while MCP-1, IL-6 and IL-1beta were studied by real-time PCR. Reactive oxygen species (ROS) was investigated by flow cytometry. Morphometric analyses were performed at 6, 24, 48 and 120 h after reperfusion. Urea peaked at 24 h, being significantly lower in knockout animals (wt = 264.4 +/- 85.21 mg/dl vs. gal-3 KO = 123.74 +/- 29.64 mg/dl, P = 0.001). Galectin-3 knockout animals presented less acute tubular necrosis and a more prominent tubular regeneration when compared with controls concurrently with lower expression of MCP-1, IL-6, IL-1beta, less macrophage infiltration and lower ROS production at early time points. Galectin-3 seems to play a role in renal IRI involving the secretion of macrophage-related chemokine, pro-inflammatory cytokines and ROS production.

• Authors: Ana Paula Fernandes Bertocchi, Gabriela Campanhole, Pamella Huey Mei Wang, Giselle Martins Gonçalves, Márcio José Damião, Marcos Antônio Cenedeze, Felipe Caetano Beraldo, Vicente de Paula Antunes Teixeira, Marlene Antônia Dos Reis, Marilda Mazzali, Alvaro Pacheco-Silva, Niels Olsen Saraiva Câmara
• Journal: Transplant international : official journal of the European Society for Organ Transplantation (Transpl Int) Vol. 21 Issue 10 Pg. 999-1007 (Oct 2008) ISSN: 0934-0874 [Print] Switzerland
• PMID: 18657091 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Autoantigens
• Biomarkers
• Ccl2 protein, mouse
• Chemokine CCL2
• Galectin 3
• Interleukin-1beta
• Interleukin-6
• Reactive Oxygen Species
• DNA

Topics

• Animals
• Autoantigens
• Biomarkers
• Chemokine CCL2 (biosynthesis, genetics)
• DNA (genetics)
• Disease Models, Animal
• Flow Cytometry
• Follow-Up Studies
• Galectin 3 (biosynthesis, genetics)
• Gene Expression
• Immunohistochemistry
• Interleukin-1beta (biosynthesis, genetics)
• Interleukin-6 (biosynthesis, genetics)
• Kidney (blood supply)
• Kidney Transplantation (pathology)
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Knockout
• Polymerase Chain Reaction
• Reactive Oxygen Species (metabolism)
• Reperfusion Injury (metabolism, pathology)