Abstract |
The tumor suppressor and transcription factor p53 is a key modulator of cellular stress responses and can trigger apoptosis in many cell types, including neurons. In this study, we have shown that the Microtubule-Associated Protein 1B ( MAP1B) light chain can interact with the tumor suppressor p53. We also demonstrate that both p53 and the MAP1B light chain (MAP1B-LC1) alter their localization from the cytoplasm to the nucleus when neuroblastoma cells, SH-SY5Y, are treated with doxorubicin. Additionally, we demonstrate that the MAP1B-LC1 negatively regulates p53-dependent transcriptional activity of a reporter construct driven by the p21 promoter. Consequently, MAP1B-LC1 binds to p53 and this interaction leads to the inhibition of doxorubicin-induced apoptosis in SH-SY5Y cells.
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Authors | So-Youn Lee, Jung-Woong Kim, Mi-Hee Jeong, Joo-Hee An, Sang-Min Jang, Ki-Hyun Song, Kyung-Hee Choi |
Journal | FEBS letters
(FEBS Lett)
Vol. 582
Issue 19
Pg. 2826-32
(Aug 20 2008)
ISSN: 0014-5793 [Print] England |
PMID | 18656471
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibiotics, Antineoplastic
- MAP1B protein, human
- Microtubule-Associated Proteins
- Tumor Suppressor Protein p53
- Doxorubicin
- Luciferases
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Topics |
- Antibiotics, Antineoplastic
(pharmacology)
- Apoptosis
- Cell Line, Tumor
- Cell Nucleus
(metabolism)
- Cytoplasm
(metabolism)
- Doxorubicin
(pharmacology)
- Genes, Reporter
- Humans
- Luciferases
(genetics)
- Microtubule-Associated Proteins
(metabolism)
- Neuroblastoma
(genetics, metabolism)
- Protein Structure, Tertiary
- Protein Transport
- Transcription, Genetic
- Transcriptional Activation
- Tumor Suppressor Protein p53
(antagonists & inhibitors, genetics, metabolism)
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