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Microtubule-associated protein 1B light chain (MAP1B-LC1) negatively regulates the activity of tumor suppressor p53 in neuroblastoma cells.

Abstract
The tumor suppressor and transcription factor p53 is a key modulator of cellular stress responses and can trigger apoptosis in many cell types, including neurons. In this study, we have shown that the Microtubule-Associated Protein 1B (MAP1B) light chain can interact with the tumor suppressor p53. We also demonstrate that both p53 and the MAP1B light chain (MAP1B-LC1) alter their localization from the cytoplasm to the nucleus when neuroblastoma cells, SH-SY5Y, are treated with doxorubicin. Additionally, we demonstrate that the MAP1B-LC1 negatively regulates p53-dependent transcriptional activity of a reporter construct driven by the p21 promoter. Consequently, MAP1B-LC1 binds to p53 and this interaction leads to the inhibition of doxorubicin-induced apoptosis in SH-SY5Y cells.
AuthorsSo-Youn Lee, Jung-Woong Kim, Mi-Hee Jeong, Joo-Hee An, Sang-Min Jang, Ki-Hyun Song, Kyung-Hee Choi
JournalFEBS letters (FEBS Lett) Vol. 582 Issue 19 Pg. 2826-32 (Aug 20 2008) ISSN: 0014-5793 [Print] England
PMID18656471 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibiotics, Antineoplastic
  • MAP1B protein, human
  • Microtubule-Associated Proteins
  • Tumor Suppressor Protein p53
  • Doxorubicin
  • Luciferases
Topics
  • Antibiotics, Antineoplastic (pharmacology)
  • Apoptosis
  • Cell Line, Tumor
  • Cell Nucleus (metabolism)
  • Cytoplasm (metabolism)
  • Doxorubicin (pharmacology)
  • Genes, Reporter
  • Humans
  • Luciferases (genetics)
  • Microtubule-Associated Proteins (metabolism)
  • Neuroblastoma (genetics, metabolism)
  • Protein Structure, Tertiary
  • Protein Transport
  • Transcription, Genetic
  • Transcriptional Activation
  • Tumor Suppressor Protein p53 (antagonists & inhibitors, genetics, metabolism)

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