Abstract | OBJECTIVE: METHOD: Microsatellite stability status was determined in resected tumour samples. Microsatellite stable (MSS) tumour samples were stratified by DNA ploidy status, as determined by flow cytometry into aneuploid MSS (CSI) and diploid MSS (MACS) cancers. Lymphocyte proliferation, quantified by bromodeoxyuridine incorporation assays assessed tumour protein immunogenicity and ELISA assays quantified inflammatory cytokine release. Kaplan-Meier survival curves and multivariate analyses were used to determine prognostic value. RESULTS: Patients with MSI-H colorectal cancer had improved outcome but those with MACS cancers undergoing curative surgery had significantly poorer disease-free survival (P = 0.002). The MACS phenotype was an independent predictor of poor outcome (HR = 2.44, 1.33-4.47, P = 0.004). Lymphocyte proliferation assays confirmed enhanced immunogenicity of MSI-H proteins and reduced immunogenicity of MACS proteins (P < 0.0001). In vitro levels of IFN-gamma (P = 0.004) and IL-18 (P < 0.0001) mirrored these differences in lymphocyte activity. CONCLUSIONS: Stratification of colorectal cancer by MSI and ploidy status may have prognostic value in patients undergoing curative surgery. MSI-H cancers display enhanced immunogenic properties but the immune response to MACS cancers appears to be absent and this may contribute to their poor prognosis.
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Authors | A Banerjea, R E Hands, M P Powar, S A Bustin, S Dorudi |
Journal | Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland
(Colorectal Dis)
Vol. 11
Issue 6
Pg. 601-8
(Jul 2009)
ISSN: 1463-1318 [Electronic] England |
PMID | 18637931
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Aged
- Aged, 80 and over
- Aneuploidy
- Cell Proliferation
- Chromosomal Instability
(genetics, immunology)
- Colorectal Neoplasms
(genetics, immunology)
- Diploidy
- Disease-Free Survival
- Female
- Follow-Up Studies
- Humans
- Immunogenetic Phenomena
- Kaplan-Meier Estimate
- Lymphocytes
(immunology)
- Male
- Microsatellite Instability
- Middle Aged
- Neoplasm Recurrence, Local
(genetics, immunology)
- Phenotype
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