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Liquid chromatography-tandem mass spectrometric quantification of the dehydration product of tetranor PGE-M, the major urinary metabolite of prostaglandin E(2) in human urine.

Abstract
A LC-MS/MS method has been developed to analyze tetranor PGE-M, the major urinary metabolite of PGE(2), that involves the acid-catalyzed dehydration of tetranor PGE-M and its deuterated (d(6)) analog followed by LC-MS/MS measurement of the dehydrated tetranor PGE-M product (tetranor PGA-M). We also report a method for quantification of creatinine in urine by LC-MS/MS to normalize tetranor PGE-M concentrations with that of urinary creatinine. These methods were used to study the effect of aspirin on urinary tetranor PGE-M levels in healthy male volunteers. Aspirin did not affect urinary creatinine concentrations but decreased urinary tetranor PGE-M concentrations by approximately 44%.
AuthorsJason R Neale, Brian J Dean
JournalJournal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 871 Issue 1 Pg. 72-7 (Aug 01 2008) ISSN: 1570-0232 [Print] Netherlands
PMID18632314 (Publication Type: Journal Article, Validation Study)
Chemical References
  • Prostaglandins
  • tetranor prostaglandin M
  • Creatinine
  • Dinoprostone
  • Aspirin
Topics
  • Adult
  • Aspirin (pharmacology)
  • Chromatography, Liquid (methods)
  • Creatinine (urine)
  • Dinoprostone (metabolism, urine)
  • Drug Stability
  • Humans
  • Male
  • Prostaglandins (urine)
  • Reproducibility of Results
  • Tandem Mass Spectrometry (methods)

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