Abstract | BACKGROUND: OBJECTIVE: METHODS:
Methacholine-induced AHR was assessed by head-out body plethysmography of wild type (WT) and TRPC6(-/-) mice. Experimental airway inflammation was induced by intraperitoneal ovalbumin (OVA) sensitization, followed by OVA aerosol challenges. Allergic inflammation and mucus production were analysed 24 h after the last allergen challenge. RESULTS:
Methacholine-induced AHR and agonist-induced contractility of tracheal rings were increased in TRPC6(-/-) mice compared with WT mice, most probably due to compensatory up-regulation of TRPC3 in airway smooth muscle cells. Most interestingly, when compared with WT mice, TRPC6(-/-) mice exhibited reduced allergic responses after allergen challenge as evidenced by a decrease in airway eosinophilia and blood IgE levels, as well as decreased levels of T-helper type 2 (Th2) cytokines (IL-5, IL-13) in the bronchoalveolar lavage. However, lung mucus production after allergen challenge was not altered by TRPC6 deficiency. CONCLUSIONS:
TRPC6 deficiency inhibits specific allergic immune responses, pointing to an important immunological function of this cation channel in Th2 cells, eosinophils, mast cells and B cells.
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Authors | S Sel, B R Rost, A O Yildirim, B Sel, H Kalwa, H Fehrenbach, H Renz, T Gudermann, A Dietrich |
Journal | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
(Clin Exp Allergy)
Vol. 38
Issue 9
Pg. 1548-58
(Sep 2008)
ISSN: 1365-2222 [Electronic] England |
PMID | 18631347
(Publication Type: Journal Article)
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Chemical References |
- Interleukin-13
- Interleukin-5
- TRPC Cation Channels
- TRPC3 cation channel
- TRPC6 Cation Channel
- Trpc6 protein, mouse
- Immunoglobulin E
- Ovalbumin
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Topics |
- Animals
- Bronchial Hyperreactivity
(immunology, metabolism, physiopathology)
- Cells, Cultured
- Epithelial Cells
(metabolism)
- Guinea Pigs
- Hypersensitivity
(immunology, metabolism, physiopathology)
- Immunoglobulin E
(blood)
- In Vitro Techniques
- Interleukin-13
(metabolism)
- Interleukin-5
(metabolism)
- Leukocytes
(pathology)
- Lung
(immunology, metabolism)
- Mice
- Mice, Knockout
- Mucus
(metabolism)
- Muscle Contraction
- Muscle, Smooth
(metabolism, physiopathology)
- Ovalbumin
(immunology)
- Pulmonary Eosinophilia
(immunology, pathology)
- TRPC Cation Channels
(biosynthesis, genetics, physiology)
- TRPC6 Cation Channel
- Trachea
(metabolism, physiopathology)
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